PXD030020

PXD030020 is an original dataset announced via ProteomeXchange.

Title	Subcellular fractionation of boar spermatozoa provides a novel view of the perinuclear theca proteome
Description	The perinuclear theca (PT) is a highly condensed, largely insoluble protein structure that surrounds the nucleus of eutherian spermatozoa. While this structure is known to be important for fertilization, little is known regarding its proteomic composition. Recent reports have indicated that the PT unexpectedly houses several somatic proteins, such as core histones, which may be important post-fertilization during re-modelling of the male pronucleus. To explore the proteomic composition of the PT we performed the first in depth, label-free proteomic characterization of the PT of boar spermatozoa. To do this, a unique subcellular fractionation protocol was first performed to isolate the PT and increase our ability to detect lowly abundant sperm proteins. Through the use of this subcellular fractionation technique we were able to quantify 1802 proteins, a result that represents unparalleled depth of coverage for the boar sperm proteome and exceeds the entire known proteome of the Sus scrofa species so far. In the PT structure itself we identified 813 proteins and confirmed the presence of previously characterized PT proteins including the core histones H2A, H2B, H3 and H4, as well as Ras-related protein Rab-2A (RAB2A) and Rab-2B (RAB2B) amongst other RAB proteins. In addition to these previously characterized PT proteins, our data also revealed that the PT is replete in proteins critical for sperm-egg fusion including: Izumo sperm-egg fusion proteins 1-4 (IZUMO1-4) and phosphoinositide phospholipase C (PLCZ1).
HostingRepository	PRIDE
AnnounceDate	2022-01-20
AnnouncementXML	Submission_2022-01-20_04:12:19.438.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Galhuber
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-28 14:14:39	ID requested
1	2022-01-20 01:26:59	announced
⏵ 2	2022-01-20 04:12:19	announced	2022-01-20: Updated project metadata.

Dataset with its publication pending

ProteomeXchange project tag: EPIC-XS

submitter keyword: Head tail division,Boar Sperm, perinuclear theca proteome

Albert J R Heck
contact affiliation	Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands
contact email	A.J.R.Heck@uu.nl
lab head
Markus Galhuber
contact affiliation	Medical University of Graz
contact email	markus.galhuber@medunigraz.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030020
     1. Label: PRIDE project
     2. Name: Subcellular fractionation of boar spermatozoa provides a novel view of the perinuclear theca proteome