PXD030015

PXD030015 is an original dataset announced via ProteomeXchange.

Title	Global regulation of cell development and aflatoxin biosynthesis in Aspergillus flavus by Afper1
Description	Aspergillus flavus contaminates crops during preharvest and post-harvest periods and produce carcinogenic mycotoxin aflatoxins posing severe threat to food safety and human health. To identify potential targets to tackle aflatoxin contamination, we have characterized a novel Afper1, sharing sequence identity with the yeast protein per1, regulating cell development and secondary metabolism in A. flavus. Notably, proteomics analysis revealed that the enzymes involved in extracellular hydrolases, conidial development, ER homeostasis, and aflatoxin biosynthesis significantly varied. Unexpectedly, enzymes participated in scavenging ROS including catA, catB, cpeB, and sodM were significantly downregulated and the accumulated ROS and sensitivity to hydrogen peroxide were confirmed by experiments. Surprisingly, Afper1 deletion significantly unregulated heterochromatin protein (HepA) and downregulated GNAT family acetyltransferase involved in heterochromatin formation. Accompanying the accumulated ROS and chromatin remodelling, enzymes participating in aflatoxins, ustiloxin B, and gliotoxin were impaired. These results implied that Afper1 affected chromatin remodelling and disturbed ER homeostasis leading to the accumulation of ROS, and ultimately resulted in growth defect and impaired secondary metabolites biosynthesis.
HostingRepository	PRIDE
AnnounceDate	2022-07-13
AnnouncementXML	Submission_2022-07-12_18:20:34.018.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xianliang Ma
SpeciesList	scientific name: Aspergillus flavus; NCBI TaxID: 5059;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-28 06:05:59	ID requested
⏵ 1	2022-07-12 18:20:34	announced

Dataset with its publication pending

submitter keyword: Aspergillus flavus

Afper1, aflatoxins, secondary metabolism

Yangyong Lv
contact affiliation	Henan University of Technology
contact email	lvyangyong@haut.edu.cn
lab head
Xianliang Ma
contact affiliation	Micrometer Biotech
contact email	maxianliangyxz@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030015
     1. Label: PRIDE project
     2. Name: Global regulation of cell development and aflatoxin biosynthesis in Aspergillus flavus by Afper1