PXD030007 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | RAW264.7 proteins interacting with Tc-MUC-1 LC-MSMS |
Description | Human toxocariasis is a neglected parasitic disease of global importance, caused predominantly by larvae of Toxocara canis. It is known that mucins secreted by larvae play crucial roles in immune recognition and evasion, but very little is understood about molecular interactions between host cells and T. canis. Here, using an integrative approach of His pull-down, mass spectrometry, co-immunoprecipitation and bioinformatics, we identified 219 proteins expressed by a murine macrophage cell line ( RAW264.7) that interact with a surface coat glycoprotein (Tc-MUC-1) of infective larvae of T. canis. Protein-protein interactions shown between Tc-MUC-1 and an actin binding protein CFL1 as well as the fatty acid binding protein FABP5 of RAW264.7 macrophages were also demonstrated in human embryonic kidney cell line (HEK 293T) expressing a mutant version of the SV40 large T antigen. |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-25 |
AnnouncementXML | Submission_2022-08-25_08:05:06.318.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | G M |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-28 05:44:22 | ID requested | |
⏵ 1 | 2022-08-25 08:05:06 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: RAW264.7 |
Tc-MUC-1 |
LC-MSMS |
Contact List
Guangxu Ma |
contact affiliation | College of Animal Sciences, Zhejiang University |
contact email | gxma1@zju.edu.cn |
lab head | |
G M |
contact affiliation | Zhejiang University |
contact email | gxma1@zju.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD030007 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030007
- Label: PRIDE project
- Name: RAW264.7 proteins interacting with Tc-MUC-1 LC-MSMS