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PXD030007

PXD030007 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRAW264.7 proteins interacting with Tc-MUC-1 LC-MSMS
DescriptionHuman toxocariasis is a neglected parasitic disease of global importance, caused predominantly by larvae of Toxocara canis. It is known that mucins secreted by larvae play crucial roles in immune recognition and evasion, but very little is understood about molecular interactions between host cells and T. canis. Here, using an integrative approach of His pull-down, mass spectrometry, co-immunoprecipitation and bioinformatics, we identified 219 proteins expressed by a murine macrophage cell line ( RAW264.7) that interact with a surface coat glycoprotein (Tc-MUC-1) of infective larvae of T. canis. Protein-protein interactions shown between Tc-MUC-1 and an actin binding protein CFL1 as well as the fatty acid binding protein FABP5 of RAW264.7 macrophages were also demonstrated in human embryonic kidney cell line (HEK 293T) expressing a mutant version of the SV40 large T antigen.
HostingRepositoryPRIDE
AnnounceDate2022-08-25
AnnouncementXMLSubmission_2022-08-25_08:05:06.318.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterG M
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-28 05:44:22ID requested
12022-08-25 08:05:06announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: RAW264.7
Tc-MUC-1
LC-MSMS
Contact List
Guangxu Ma
contact affiliationCollege of Animal Sciences, Zhejiang University
contact emailgxma1@zju.edu.cn
lab head
G M
contact affiliationZhejiang University
contact emailgxma1@zju.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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