PXD030002

PXD030002 is an original dataset announced via ProteomeXchange.

Title	Responses of macrophages to repeated exposures to different forms of amorphous silica
Description	Synthetic amorphous silica (SAS) is a nanomaterial used in a wide variety of applications, including the use as a food additive. Two types of SAS are commonly employed as a powder additive, precipitated silica and fumed silica. Numerous studies have investigated the effects of synthetic amorphous silica on mammalian cells. However, most of them have used an exposure scheme based on a single dose of SAS. In this study, we have used instead a repeated 10-days exposure scheme, closer to the occupational exposure encountered in daily life. As a biological model we have used the murine macrophage cell line J774A.1, as macrophages are very important innate immune cells in the response to particulate materials. In order to get a better appraisal of the macrophage responses to this repeated exposure to SAS, we have used proteomics as a wide-scale approach. Furthermore, some of the biological pathways detected as modulated by the exposure to SAS by the proteomic experiments have been validated through targeted experiments. Overall, proteomics showed that precipitated SAS induced a more important macrophage response than fumed SAS at equal dose. Nevertheless, validation experiments showed that most of the responses detected by proteomics are indeed adaptive, as the cellular homeostasis appeared to be maintained at the end of the exposure. For example, the intracellular glutathione levels or the mitochondrial transmembrane potential at the end of the 10 days exposure were similar for SAS-exposed cells and for unexposed cells. Nevertheless, important functions of macrophages such as phagocytosis, TNF and interleukin-6 secretion were up-modulated after exposure, as was the expression of important membrane proteins such as the scavenger receptor or the MAC-1 receptor. These results suggest that repeated exposure to low doses of SAS slightly modulates the immune functions of macrophages, which may alter the homeostasis of the immune system.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_04:49:03.745.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030002
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hélène Diemer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-28 05:12:05	ID requested
⏵ 1	2022-06-09 04:49:04	announced

Torres A, Collin-Faure V, Diemer H, Moriscot C, Fenel D, Gallet B, Cianf, é, rani S, Sergent JA, Rabilloud T, Repeated Exposure of Macrophages to Synthetic Amorphous Silica Induces Adaptive Proteome Changes and a Moderate Cell Activation. Nanomaterials (Basel), 12(9):(2022) [pubmed]

submitter keyword: Mouse, macrophage, LC-MS/MS

Sarah Cianferani
contact affiliation	Laboratoire de Spectrométrie de Masse BioOrganique (LSMBO), Université de Strasbourg,CNRS, IPHC UMR 7178, 67087 Strasbourg,France
contact email	sarah.cianferani@unistra.fr
lab head
Hélène Diemer
contact affiliation	LSMBO
contact email	hdiemer@unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030002
     1. Label: PRIDE project
     2. Name: Responses of macrophages to repeated exposures to different forms of amorphous silica