PXD029995

PXD029995 is an original dataset announced via ProteomeXchange.

Title	Characterization of differentially abundant proteins among Leishmania (Viannia) braziliensis strains isolated from atypical or typical lesions
Description	Leishmania (Viannia) braziliensis is the main etiological agent of cutaneous and mucocutaneous leishmaniasis in Latin America. Reports have described non-ulcerated atypical tegumentary leishmaniasis cases caused by L. braziliensis in several regions of the world, including in patients from the Xacriabá Indigenous reserve, in São João das Missões/Minas Gerais - Brazil. Parasites isolated from these atypical clinical lesions have previously been found to be resistant to antimony-based therapeutics. In the present study, proteins displaying differential abundance in 2 strains of L. braziliensis isolated from patients with atypical lesions compared with 4 strains isolated from patients with typical lesions were identified using a quantitative proteomics approach based on tandem mass tag labeling (TMT) and mass spectrometry. A total of 532 (p value <0.05) differentially abundant proteins were identified (298 up-regulated and 234 down-regulated) in strains from atypical lesions compared to strains from typical lesions. We observed a variety of proteins with differential abundance among the studied strains. Prominent positively regulated in atypical strains included proteins which may confer a greater survival inside the macrophage, proteins related to resistance to antimony and higher peroxidase activity. Also were identified proteins suggest as new drug and vaccines target. Our data contribute to characterization of these intriguing L. braziliensis strains, and sheds new light on ACL cases has been associated with therapeutic failures.
HostingRepository	PRIDE
AnnounceDate	2022-02-11
AnnouncementXML	Submission_2022-02-11_05:05:46.774.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bárbara Esteves
SpeciesList	scientific name: Leishmania braziliensis; NCBI TaxID: 5660;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-28 04:23:47	ID requested
⏵ 1	2022-02-11 05:05:47	announced

Dataset with its publication pending

submitter keyword: Leishmania braziliensis, atypical, proteome, abundance, label based, TMT.

Hélida Monteiro de Andrade
contact affiliation	Department of Parasiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte-MG, Brazil.
contact email	helidandrade@gmail.com
lab head
Bárbara Esteves
contact affiliation	UFMG
contact email	barbarabeiral@yahoo.com.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD029995

PRIDE project URI

• PRIDE
  1. PXD029995
     1. Label: PRIDE project
     2. Name: Characterization of differentially abundant proteins among Leishmania (Viannia) braziliensis strains isolated from atypical or typical lesions