PXD029992

PXD029992 is an original dataset announced via ProteomeXchange.

Title	Antibody repertoire sequencing reveals systemic and mucosal immunosenescence in the short-lived turquoise killifish
Description	Aging individuals exhibit a pervasive decline in adaptive immune function, with important implications for health and lifespan. Previous studies have found a pervasive loss of immune-repertoire diversity in human peripheral blood; however, little is known about repertoire aging in other immune compartments, or in species other than humans. Here, we perform the first study of immune-repertoire aging in an emerging model of vertebrate aging, the African turquoise killifish (Nothobranchius furzeri). Despite their extremely short lifespans, these killifish exhibit complex and individualised heavy-chain repertoires, with a generative process capable of producing millions of distinct productive sequences. Whole-body killifish repertoires decline rapidly in within-individual diversity with age, while between-individual variability increases. Large, expanded B-cell clones exhibit far greater diversity loss with age than small clones, suggesting an important difference in the age-sensitivity of different B cell populations. Compared to the whole body, the immune repertoires of isolated intestinal samples exhibit much more dramatic age-related phenotypes, apparently due to an elevated prevalence of age-sensitive expanded clones. Our results highlight the importance of organ-specific dynamics in adaptive immunosenescence.
HostingRepository	PRIDE
AnnounceDate	2026-03-30
AnnouncementXML	Submission_2026-03-29_16:20:59.423.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilian Atanassov
SpeciesList	scientific name: Nothobranchius furzeri; NCBI TaxID: NEWT:105023;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-28 03:45:11	ID requested
⏵ 1	2026-03-29 16:21:00	announced

10.1038/s43587-026-01086-2;

Morabito G, D, ö, nertas HM, Sperti L, Seidel J, Poursadegh Zonouzi A, Poeschla M, Valenzano DR, Spontaneous aging-associated inflammation and genome instability in the immune system of turquoise killifish. Nat Aging, 6(3):665-681(2026) [pubmed]

submitter keyword: immunoglobulins,aging, B cells, immunosenescence

Dario Valenzano
contact affiliation	Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Str. 9b, 50931 Cologne, GERMANY
contact email	dario.valenzano@age.mpg.de
lab head
Ilian Atanassov
contact affiliation	Max Planck Institute for Biology of Aging
contact email	ilian.atanassov@age.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD029992

PRIDE project URI

• PRIDE
  1. PXD029992
     1. Label: PRIDE project
     2. Name: Antibody repertoire sequencing reveals systemic and mucosal immunosenescence in the short-lived turquoise killifish