PXD029981 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Targeting mitochondrial translation overcomes multidrug resistance in FBXW7-deficient cells |
Description | FBXW7 is one of the most frequently mutated tumor suppressors, the deficiency of which has been associated with resistance to some anticancer therapies. Through bioinformatic analyses and genome-wide CRISPR screens, we here reveal that FBXW7 deficiency leads to multi-drug resistance (MDR), to a bigger extent than well-established MDR-drivers such as ABCB1. Proteomic data from FBXW7-deficient cells and human cancer samples identify the upregulation of mitochondrial function as a hallmark of FBXW7 deficiency, which has been previously linked to an increased resistance to chemotherapy. Accordingly, genetic or chemical targeting of mitochondria is preferentially toxic for FBXW7-deficient cells. For instance, targeting mitochondrial translation with the antibiotic Tigecycline efficiently kills FBXW7-deficient cells in vitro and in vivo, by a mechanism that involves activation of the Integrated Stress Response (ISR). Searching for additional drugs that overcome MDR in FBXW7-deficient cells, we found several targeted therapies such as Erlotinib, Dasatinib or Vemurafenib which unexpectedly also activate the ISR. Together, our study reveals that one of the most frequent mutations in cancer reduces the sensitivity to the vast majority of available therapies, and identifies a general principle to overcome such resistance. |
HostingRepository | PRIDE |
AnnounceDate | 2022-06-08 |
AnnouncementXML | Submission_2022-06-08_07:26:13.272.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Eduardo Zarzuela |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-26 08:24:05 | ID requested | |
⏵ 1 | 2022-06-08 07:26:13 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: mESCs, TMT, Q Exactive |
Contact List
Javier Muñoz |
contact affiliation | Proteomics Unit, Biotechnology Programme, CNIO (Centro Nacional de Investigaciones Oncológicas),Madrid, Spain |
contact email | javier.munozperalta@osakidetza.eus |
lab head | |
Eduardo Zarzuela |
contact affiliation | Centro Nacional de Investigaciones Oncológicas |
contact email | eduzarfer@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD029981 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029981
- Label: PRIDE project
- Name: Targeting mitochondrial translation overcomes multidrug resistance in FBXW7-deficient cells