PXD029967

PXD029967 is an original dataset announced via ProteomeXchange.

Title	SARS-CoV-2 Spike Protein and Murine Hepatitis Virus Inhib-its S. pneumoniae and S. aureus Biofilms
Description	The recovery of other pathogens in COVID-19 patients has been reported. The presence of either co-infection or superinfection with bacterial pathogens was associated with poor outcomes, including increased mortality. The recognition of possibility of SARS-CoV-2 co-infection is important as it enables the implementation of appropriate infection control measures and therapy. This is a proof-of-concept study uses in vitro approaches (including crystal violet assay, microrheology, and LC-MS-based prote-omics) to investigate the interaction between SARS-CoV-2 and biofilms of bacteria (S. pneumoniae and S. aureus). SARS-CoV-2 spike protein S1 subunit was found to suppress biofilm formation of both bacteria. The effect of coronavirus and spike protein on bac-terial biofilm was supported by proteomics data that shows variations in proteins in-volved in quorum sensing and biofilm formation/maturation. Preliminary in vitro data suggest that dispersion of opportunistic pathogens from biofilm may be associated with poor prognosis in co-infections. However, further investigations are needed to establish bacterial biofilm as a risk factor for secondary pneumonia in COVID-19 patients.
HostingRepository	PRIDE
AnnounceDate	2022-05-25
AnnouncementXML	Submission_2022-05-25_09:12:51.742.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mun Fai Loke
SpeciesList	scientific name: Streptococcus pneumoniae; NCBI TaxID: 1313; scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	methylthiolated residue; monohydroxylated residue; acetylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-26 04:56:36	ID requested
⏵ 1	2022-05-25 09:12:52	announced

Loke MF, Yadav I, Lim TK, van der Maarel JRC, Sham LT, Chow VT, . Int J Mol Sci, 23(6):(2022) [pubmed]

submitter keyword: Streptococcus pneumoniae

Staphylococcus aureus

biofilm

SARS-CoV-2

spike protein

Vincent Chow
contact affiliation	Associate Professor, Department of Microbiology and Immunology
contact email	micctk@nus.edu.sg
lab head
Mun Fai Loke
contact affiliation	National University of Singapore
contact email	lmunfai@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029967

PRIDE project URI

• PRIDE
  1. PXD029967
     1. Label: PRIDE project
     2. Name: SARS-CoV-2 Spike Protein and Murine Hepatitis Virus Inhib-its S. pneumoniae and S. aureus Biofilms