PXD029966

PXD029966 is an original dataset announced via ProteomeXchange.

Title	Establishment of fetomaternal tolerance via glycan-mediated B cell suppression
Description	The immune system must be able to distinguish self from non-self. During pregnancy, the mother’s immune system does not recognize the placenta as foreign because proteins expressed by tropbholasts, the placental cells that interface with the maternal immune system, do not activate maternal T cells. These activation defects have been previously attributed to suppression by regulatory T cells, while mechanisms of maternal B cell tolerance to trophoblast antigens have not been identified. In this study, we provide evidence that glycan-mediated B cell suppression plays a key role in establishing fetomaternal tolerance in mice. We find that trophoblast antigen-specific B cells are profoundly suppressed via CD22/LYN inhibitory signaling, in turn implicating the antigens’ sialic acids as key suppressive determinants. We also find that B cells mediate the antigen’s MHCII-restricted presentation to CD4 T cells, leading to T cell suppression. The specific goal of the mass spectrometry undertaking deposited here was to identify sialylated “true” placental-derived proteins present in the human and mouse serum proteome during pregnancy. Overall, our findings reveal protein glycosylation as a fundamental feature of placental “self-recognition” and may have relevance to pregnancy complications and tumor immune evasion. Furthermore, we anticipate these findings will enhance synthetic efforts to harness glycans to control antigen-specific immune responses in the treatment of autoimmune diseases.
HostingRepository	PRIDE
AnnounceDate	2022-01-12
AnnouncementXML	Submission_2022-03-18_07:12:40.520.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sami Tuomivaara
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N-acetylneuraminylated residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-26 04:47:21	ID requested
1	2022-01-12 13:28:27	announced
⏵ 2	2022-03-18 07:12:41	announced	2022-03-18: Updated publication reference for PubMed record(s): 35236989.

Rizzuto G, Brooks JF, Tuomivaara ST, McIntyre TI, Ma S, Rideaux D, Zikherman J, Fisher SJ, Erlebacher A, cell suppression. Nature, 603(7901):497-502(2022) [pubmed]

submitter keyword: Placenta glycoproteins, sialic acid, B cells, CD22, fetomaternal tolerance, LC-MSMS

Susan J Fisher
contact affiliation	Department of Obstetrics, Gynecology, and Reproductive Sciences, Center for Reproductive Sciences, University of California, San Francisco, CA 94143, USA Eli and Edythe Broad Center for Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA 94143, USA Department of Anatomy, University of California, San Francisco, CA 94143, USA
contact email	susan.fisher@ucsf.edu
lab head
Sami Tuomivaara
contact affiliation	University of California, San Francisco
contact email	sami.tuomivaara@ucsf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD029966

PRIDE project URI

• PRIDE
  1. PXD029966
     1. Label: PRIDE project
     2. Name: Establishment of fetomaternal tolerance via glycan-mediated B cell suppression