PXD029959

PXD029959 is an original dataset announced via ProteomeXchange.

Title	Differential scaling between G1 protein production and cell size dynamics promotes commitment to the cell division cycle in budding yeast
Description	In the unicellular eukaryote Saccharomyces cerevisiae, Cln3–cyclin-dependent kinase activity enables Start, the irreversible commitment to the cell division cycle. However, the concentration of Cln3 has been paradoxically considered to remain constant during G1, due to the presumed scaling of its production rate with cell size dynamics. Measuring metabolic and biosynthetic activity during cell cycle progression in single cells, we found that cells exhibit pulses in their protein production rate. Rather than scaling with cell size dynamics, these pulses follow the intrinsic metabolic dynamics, peaking around Start. Using a viral- based bicistronic construct and targeted proteomics to measure Cln3 at the single-cell and population levels, we show that the differential scaling between protein production and cell size leads to a temporal increase in Cln3 concentration, and passage through Start. This differential scaling causes Start in both daughter and mother cells across growth conditions. Thus, uncou- pling between two fundamental physiological parameters drives cell cycle commitment.
HostingRepository	PRIDE
AnnounceDate	2022-05-16
AnnouncementXML	Submission_2022-05-16_05:28:00.869.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029959
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schmidt
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-26 04:09:41	ID requested
1	2022-05-16 05:25:33	announced
⏵ 2	2022-05-16 05:28:01	announced	2022-05-16: Updated publication reference for PubMed record(s): 35482514.

Litsios A, Goswami P, Terpstra HM, Coffin C, Vuillemenot LA, Rovetta M, Ghazal G, Guerra P, Buczak K, Schmidt A, Tollis S, Tyers M, Royer CA, Milias-Argeitis A, Heinemann M, The timing of Start is determined primarily by increased synthesis of the Cln3 activator rather than dilution of the Whi5 inhibitor. Mol Biol Cell, 33(5):rp2(2022) [pubmed]

submitter keyword: Yeast, LC-MS, cell division, phosphorylation

Alexander Schmidt
contact affiliation	PCF, Biozentrum, University of Basel, Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliation	Proteomics Core Facility
contact email	alex.schmidt@unibas.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD029959

PRIDE project URI

• PRIDE
  1. PXD029959
     1. Label: PRIDE project
     2. Name: Differential scaling between G1 protein production and cell size dynamics promotes commitment to the cell division cycle in budding yeast