PXD029952

PXD029952 is an original dataset announced via ProteomeXchange.

Title	Cas1 and Fen1 Display Commutable Functions During Archaeal DNA Damage Repair
Description	CRISPR-Cas constitutes an adaptive prokaryotic defence system against invasive nucleic acids like viruses and plasmids. Beyond their role in immunity, CRISPR-Cas systems have been shown to closely interact with components of cellular DNA repair pathways either by regulating their expression or via direct protein-protein contact or enzymatic activity. The integrase Cas1 is usually involved in the adaptation phase of CRISPR-Cas immunity but its function in cellular DNA repair pathways has been proposed before. Here, we analysed the capacity of an archaeal Cas1 from Haloferax volcanii to compensate DNA damage induced by oxidative stress and found that a deletion of the cas1 gene led to severe growth defects after stress induction. In addition, our results indicate that Cas1 is directly involved in DNA damage repair as the enzymatically active site of the protein is crucial for growth rescue under oxidative conditions. Based on biochemical cleavage assays, we propose a mechanism in which Cas1 exerts a similar function like the DNA repair protein Fen1 by resolving branched repair intermediate structures. Overall, the present study broadens our understanding of the functional link between CRISPR-Cas immunity and DNA repair by demonstrating that Cas1 and Fen1 display commutable roles during archaeal DNA damage repair.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_06:23:38.912.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Henning Urlaub
SpeciesList	scientific name: Haloferax volcanii DS2; NCBI TaxID: 309800;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-26 03:26:05	ID requested
⏵ 1	2022-05-20 06:23:39	announced

W, ö, rtz J, Smith V, Fallmann J, K, ö, nig S, Thuraisingam T, Walther P, Urlaub H, Stadler PF, Allers T, Hille F, Marchfelder A, Cas1 and Fen1 Display Equivalent Functions During Archaeal DNA Repair. Front Microbiol, 13():822304(2022) [pubmed]

submitter keyword: CRISPR, Cas1, Fen1, archaea, DNA damage

Prof. Dr. Henning Urlaub
contact affiliation	Max-Planck Institute for Biophysical Chemistry
contact email	henning.urlaub@mpibpc.mpg.de
lab head
Henning Urlaub
contact affiliation	Max-Plank Institute for Biophysical Chemistry
contact email	msbio.goettingen@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029952
     1. Label: PRIDE project
     2. Name: Cas1 and Fen1 Display Commutable Functions During Archaeal DNA Damage Repair