PXD029952 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cas1 and Fen1 Display Commutable Functions During Archaeal DNA Damage Repair |
Description | CRISPR-Cas constitutes an adaptive prokaryotic defence system against invasive nucleic acids like viruses and plasmids. Beyond their role in immunity, CRISPR-Cas systems have been shown to closely interact with components of cellular DNA repair pathways either by regulating their expression or via direct protein-protein contact or enzymatic activity. The integrase Cas1 is usually involved in the adaptation phase of CRISPR-Cas immunity but its function in cellular DNA repair pathways has been proposed before. Here, we analysed the capacity of an archaeal Cas1 from Haloferax volcanii to compensate DNA damage induced by oxidative stress and found that a deletion of the cas1 gene led to severe growth defects after stress induction. In addition, our results indicate that Cas1 is directly involved in DNA damage repair as the enzymatically active site of the protein is crucial for growth rescue under oxidative conditions. Based on biochemical cleavage assays, we propose a mechanism in which Cas1 exerts a similar function like the DNA repair protein Fen1 by resolving branched repair intermediate structures. Overall, the present study broadens our understanding of the functional link between CRISPR-Cas immunity and DNA repair by demonstrating that Cas1 and Fen1 display commutable roles during archaeal DNA damage repair. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-20 |
AnnouncementXML | Submission_2022-05-20_06:23:38.912.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Henning Urlaub |
SpeciesList | scientific name: Haloferax volcanii DS2; NCBI TaxID: 309800; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-26 03:26:05 | ID requested | |
⏵ 1 | 2022-05-20 06:23:39 | announced | |
Publication List
W, ö, rtz J, Smith V, Fallmann J, K, ö, nig S, Thuraisingam T, Walther P, Urlaub H, Stadler PF, Allers T, Hille F, Marchfelder A, Cas1 and Fen1 Display Equivalent Functions During Archaeal DNA Repair. Front Microbiol, 13():822304(2022) [pubmed] |
Keyword List
submitter keyword: CRISPR, Cas1, Fen1, archaea, DNA damage |
Contact List
Prof. Dr. Henning Urlaub |
contact affiliation | Max-Planck Institute for Biophysical Chemistry |
contact email | henning.urlaub@mpibpc.mpg.de |
lab head | |
Henning Urlaub |
contact affiliation | Max-Plank Institute for Biophysical Chemistry |
contact email | msbio.goettingen@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029952
- Label: PRIDE project
- Name: Cas1 and Fen1 Display Commutable Functions During Archaeal DNA Damage Repair