PXD029944

PXD029944 is an original dataset announced via ProteomeXchange.

Title	Identification of protein-protein interaction mediated by N-myristoylated proteins using photocrosslinkable clickable myristoylation probes X3 and X10
Description	HeLa cells were treated with myristoylation probes X3 and X10, which incorporate both alkyne and diazirine moieties. Cells were then treated with NMT ihibitor IMP-366 (DDD85464) or DMSO vehicle and UV-irradiated before lysis. The experiment identifies myristoylated proteins through comparison of vehicle and NMT inhibitor treated samples. A number of non-myristoylated proteins were enriched together with myristoylated proteins indicating possible photocrosslinked protein interactors.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:54:27.033.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roman Fedoryshchak
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N-myristoylglycine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-26 03:24:51	ID requested
1	2023-02-04 06:12:32	announced
⏵ 2	2023-11-14 08:54:27	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: diazirine, NMT, photocrosslinking,myristoylaion

Ed Tate
contact affiliation	Professor of Chemical Biology, Imperial College London Satellite Group Leader, Francis Crick Institute Founder & SAB, Myricx Pharma Ltd Room 301B, Molecular Sciences Research Hub, White City Campus 82 Wood Lane, London W12 0BZ, UK
contact email	e.tate@imperial.ac.uk
lab head
Roman Fedoryshchak
contact affiliation	The Francis Crick Institute
contact email	roman.fedoryshchak@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD029944

PRIDE project URI

• PRIDE
  1. PXD029944
     1. Label: PRIDE project
     2. Name: Identification of protein-protein interaction mediated by N-myristoylated proteins using photocrosslinkable clickable myristoylation probes X3 and X10