PXD029936

PXD029936 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide cellular thermal shift assay reveals novel crosstalk between brassinosteroid and auxin signaling
Description	We demonstrated that bikinin changed the thermal stability of some of its target proteins as well as several known or putative interacting proteins of Arabidopsis GSK3s. By combining the thermal- and the phospho-proteomes of bikinin we uncovered the auxin carrier PIN-FORMED1 (PIN1) as a novel substrate of the Arabidopsis GSK3s. We showed that inhibition the kinase activity of GSK3s by brassinosteroids or the specific chemical inhibitor bikinin led to the loss of PIN1 polarity, revealing a novel crosstalk between brassinosteroid and auxin signaling. Hence, our study demonstrates the applicability of CETSA MS in plant intact cells for identification of small-molecule targets and for discovery of novel protein-protein interactions.
HostingRepository	PRIDE
AnnounceDate	2022-03-08
AnnouncementXML	Submission_2022-03-08_00:37:36.311.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiangyu Xu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-26 03:15:50	ID requested
⏵ 1	2022-03-08 00:37:36	announced

Dataset with its publication pending

submitter keyword: Arabidopsis cell suspension, chemical genetics, brassinosteroids, auxin，MS/MS spectra

Ive DeSmet
contact affiliation	Ive De Smet - Group leader Functional Phosphoproteomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium www.psb.ugent.be
contact email	Ive.DeSmet@psb.ugent.be
lab head
Xiangyu Xu
contact affiliation	PSB-VIP
contact email	xiangyu.xu@psb.ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD029936

PRIDE project URI

• PRIDE
  1. PXD029936
     1. Label: PRIDE project
     2. Name: Proteome-wide cellular thermal shift assay reveals novel crosstalk between brassinosteroid and auxin signaling