PXD029931

PXD029931 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of E. coli MG1655 after removal of oriC
Description	The project aim was to describe proteomic changes in E. coli MG1655 strain after removal of oriC. Temperature-sensitive transcriptional repressor cI857 and its target promoter was used to control the expression of a serine recombinase from bacteriophage phiC31 (phiC31 integrase). cI857 is a temperature-sensitive mutant that represses its target promoter at 30°C, but the repression is relieved at 37°C. We generated an E. coli “switcher” strain in which the oriC sequence in the chromosome is flanked by phiC31-integrase-specific attP and attB sites and in this strain the oriC is irreversibly excised when cells are incubated at 37°C.
HostingRepository	PRIDE
AnnounceDate	2021-12-15
AnnouncementXML	Submission_2021-12-15_02:42:42.649.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029931
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Villu Kasari
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-26 02:03:05	ID requested
⏵ 1	2021-12-15 02:42:43	announced

Dataset with its publication pending

submitter keyword: E. coli, oriC, growth decoupling, switcher

Arvi Jõers
contact affiliation	Institute of Technology, University of Tartu, Estonia
contact email	arvi.joers@ut.ee
lab head
Villu Kasari
contact affiliation	University of Tartu
contact email	villu.kasari@ut.ee
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD029931

PRIDE project URI

• PRIDE
  1. PXD029931
     1. Label: PRIDE project
     2. Name: Proteomic analysis of E. coli MG1655 after removal of oriC