PXD029928

PXD029928 is an original dataset announced via ProteomeXchange.

Title	Differences in the abundance of auxin homeostasis proteins suggest a central role for in vitro tissue differentiation in Coffea arabica
Description	Coffea arabica is one of the most important crops worldwide. In vitro culture is an alternative for achieving Coffea regeneration, propagation, conservation, genetic improvement, and genome editing. The aim of this work is to identify proteins involved in auxin homeostasis by isobaric tandem mass tag (TMT) and the synchronous precursor selection (SPS)-based MS3 technology on the Orbitrap Fusion™ Tribrid mass spectrometer™ in three types of biological material corre-sponding to C. arabica: plantlet leaves, calli, and suspension culture. Proteins included in the β-oxidation of indole butyric acid, and in the signaling, transport, and conjugation of in-dole-3-acetic acid were identified, such as the indole butyric response (IBR), the auxin binding protein (ABP), the ATP-binding cassette transporters (ABC), the Gretchen-Hagen 3 proteins (GH3), and the indole-3-acetic-leucine resistant proteins (ILR). A more significant accumulation of pro-teins involved in auxin homeostasis was found in the suspension cultures vs the plantlet com-parison, followed by callus vs plantlet and suspension culture vs callus, suggesting greater par-ticipation of these proteins as cell differentiation increases.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_04:59:07.838.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miguel Elizalde
SpeciesList	scientific name: Coffea arabica; NCBI TaxID: 13443;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-26 02:02:44	ID requested
⏵ 1	2022-02-17 04:59:08	announced

Quintana-Escobar AO, M, é, ndez-Hern, á, ndez HA, Galaz-, Á, valos RM, Elizalde-Contreras JM, Reyes-Soria FA, Aguilar-Hern, á, ndez V, Ru, í, z-May E, Loyola-Vargas VM, . Plants (Basel), 10(12):(2021) [pubmed]

submitter keyword: LC-MS, TMT, Coffea arabica

Jose Miguel Elizalde Contreras
contact affiliation	Instituto de Ecología, A.C.
contact email	jose.elizald@inecol.mx
lab head
Miguel Elizalde
contact affiliation	Instituto de Ecolog'ia A.C.
contact email	jose.elizalde@inecol.mx
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029928
     1. Label: PRIDE project
     2. Name: Differences in the abundance of auxin homeostasis proteins suggest a central role for in vitro tissue differentiation in Coffea arabica