PXD029924
PXD029924 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | KOPI - Kinase inhibitor proteome impact analysis |
Description | Kinase inhibitors often exhibit on/off-target effects, and efficient data analysis is essential for assessing these effects on the proteome. We developed a workflow for rapidly performing such a proteomic assessment, termed as kinase inhibitor proteome impact analysis (KOPI). We demonstrate KOPI’s utility with staurosporine on the leukemic K562 cell proteome. We analysed the changes in the proteome, thermal stability and phosphoproteome in the cells response to staurosporine. We identified systemically staurosporine’s non-kinome interactors, and showed for the first time that it caused paradoxical hyper- and biphasic phosphorylation. |
HostingRepository | jPOST |
AnnounceDate | 2022-06-16 |
AnnouncementXML | Submission_2023-04-02_20:19:10.180.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Radoslaw Sobota |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; alpha-amino acetylated residue; deamidated L-glutamine; deamidated L-asparagine |
Instrument | instrument |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-11-26 01:31:52 | ID requested | |
1 | 2022-06-16 06:31:38 | announced | |
2 | 2022-09-18 02:36:10 | announced | 2022-09-18: Updated FTP location. |
⏵ 3 | 2023-04-02 20:19:11 | announced | 2023-04-03: Updated PubMed. |
Publication List
Li GX, Zhao T, Wang LC, Choi H, Lim YT, Sobota RM, KOPI: Kinase inhibitOr Proteome Impact analysis. Sci Rep, 12(1):13015(2022) [pubmed] |
Keyword List
submitter keyword: TMT, CETSA, protein expression, phosphorylation |
Contact List
Lim Yan Ting, Radoslaw Sobota | |
---|---|
lab head | |
Radoslaw Sobota | |
contact affiliation | IMCB A-STAR Singapore |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001389/ |