PXD029920

PXD029920 is an original dataset announced via ProteomeXchange.

Title	Integrated proteomics and N-glycoproteomic characterization of glioblastoma multiform revealed N-glycosylation heterogeneities and elevations in mannose, sialyation and fucosylation
Description	Glioblastoma multiforme (GBM) is a highly malignant primary brain tumor. While tremendous efforts in multi-omics research to profile the dysregulated molecular mechanisms and cellular pathways of the disease have been exerted, the glycoproteomics of GBM remains incompletely understood. Glycosylation, as one of the most important post-translational modifications, is crucial in regulating cell proliferation and relevant oncogenic pathways. In this study, we systematically profiled the N-glycoproteomics of para-cancerous and cancerous tissues from GBM patients to reveal site-specific N-glycosylation patterns defined by intact glycopeptides (IGPs). A total of 1863 distinct IGPs with 161 N-linked glycan compositions and 326 glycosites were identified and quantified. We then discovered that 396 IGPs from 43 glycoproteins differed between adjacent and GBM tissues. A more in-depth characterization of the enriched functions of differentially expressed glycopeptides was performed. Thereafter, the proteomics and glycoproteomics data were combined, and the proportion of normalized glycosylation alterations was calculated to determine whether the difference in expression of glycopeptides could be attributed to global protein levels or glycosylation. Alterations in glycosylation triggered by site-specific N-glycans and glycoprotein abundance, as well as glycosite heterogeneity, were demonstrated. Ultimately, examination of the overall glycosylation levels revealed the positive contribution of oligo-mannose resulting from altered glycol-enzyme expression levels, along with aberrant complex glycans caused by increased sialylated or/and fucosylated glycans. Overall, the dataset highlights N-glycosylation heterogeneities and altered glycans at post-translational levels and provides valuable information for novel therapeutic approaches and specific detection strategies.
HostingRepository	iProX
AnnounceDate	2021-11-26
AnnouncementXML	Submission_2021-11-25_22:00:54.124.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kaiyue Xu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-25 22:00:32	ID requested
⏵ 1	2021-11-25 22:00:54	announced

Dataset with its publication pending

submitter keyword: Glioblastoma multiform, proteomics, intact glycopeptides, N-glycosylation heterogeneities, N-glycan

Huijuan Wang
contact affiliation	School of Life Sciences, Northwest University
contact email	whj@nwu.edu.cn
lab head
Kaiyue Xu
contact affiliation	School of Life Sciences, Northwest University, Xi’an, China
contact email	bioxzy163@163.com
dataset submitter

iProX dataset URI