PXD029911

PXD029911 is an original dataset announced via ProteomeXchange.

Title	Insights on the Differential Preservation of Bone Proteomes in Inhumated and Entombed Cadavers from Italian Forensic Caseworks
Description	Bone is a long-lasting biological tissue often used in forensic investigations as it retains vital biomolecular information commonly used for identification purposes. Bone proteins have attracted interest for their potential in estimating post-mortem interval (PMI) and age-at-death (AAD). However, the preservation of such proteins is highly dependent on intrinsic and extrinsic factors, and these have an impact in the potential application of molecular techniques to forensic sciences. The present study aims at investigating the effect that two commonly used types of burial practices (entombment and inhumation) have on bone protein survival. The sample consists in 14 exhumed individuals from cemeteries in south of Italy at with different AADs (29-85 yeas) and PMIs (1-37 years). LC-MS/MS analyses show that 16 proteins are better preserved in the entombed condition and four in the inhumated one, while no clear cluster separation is detected with principal component analysis. Besides the different burial environments, several potential protein markers are identified for PMI and AAD estimation. Overall, preliminary results show that the two burial environments seem to play a marginal role in the differential preservation of non-collagenous proteins and in the accumulation of post-translational modifications, confirming the potential of LC-MS/MS based proteomics in forensic sciences.
HostingRepository	PRIDE
AnnounceDate	2022-03-25
AnnouncementXML	Submission_2022-03-25_09:02:19.052.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029911
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Noemi Procopio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-24 23:55:24	ID requested
⏵ 1	2022-03-25 09:02:19	announced

Bonicelli A, Di Nunzio A, Di Nunzio C, Procopio N, Insights into the Differential Preservation of Bone Proteomes in Inhumed and Entombed Cadavers from Italian Forensic Caseworks. J Proteome Res, 21(5):1285-1298(2022) [pubmed]

submitter keyword: Bone, Burial environment, Post-mortem interval, Age-at-death, Forensics, Post-Translational Protein Modifications

Noemi Procopio
contact affiliation	Forensic Science Research Group, Faculty of Health and Life Sciences, Applied Sciences, Northumbria University, NE1 8ST, Newcastle Upon Tyne, UK
contact email	noemi.procopio@northumbria.ac.uk
lab head
Noemi Procopio
contact affiliation	Northumbria University
contact email	noemi.procopio@northumbria.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029911
     1. Label: PRIDE project
     2. Name: Insights on the Differential Preservation of Bone Proteomes in Inhumated and Entombed Cadavers from Italian Forensic Caseworks