PXD029895

PXD029895 is an original dataset announced via ProteomeXchange.

Title	An ERAP2 inhibitor induces cell-surface presentation of many new and potentially antigenic peptides by cancer cells
Description	Recent studies have linked the activity of ER aminopeptidase 2 (ERAP2) to increased efficacy of immune-checkpoint inhibitor cancer immunotherapy, suggesting that pharmacological inhibition of ERAP2 could have important therapeutic implications. To explore the effects of ERAP2 inhibition on the immunopeptidome of cancer cells we treated MOLT4 T lymphoblast leukaemia cells with a recently developed selective ERAP2 inhibitor, isolated Major Histocompatibility class I molecules (MHCI) and sequenced bound peptides by tandem liquid chromatography mass spectrometry. Inhibitor treatment induced significant shifts on the immunopeptidome so as more than 20% of detected peptides were either novel or significantly upregulated. Most of the inhibitor-induced peptides were 9mers and had sequence motifs and predicted affinity consistent with being optimal ligands for at least one of the MHCI alleles carried by MOLT4 cells. Such inhibitor-induced peptides could serve as triggers for novel cytotoxic responses against cancer cells and synergize with the therapeutic effect of immune-checkpoint inhibitors.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_04:35:52.410.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Samiotaki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deaminated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-24 05:50:34	ID requested
⏵ 1	2022-02-22 04:35:53	announced

Temponeras I, Stamatakis G, Samiotaki M, Georgiadis D, Pratsinis H, Panayotou G, Stratikos E, ERAP2 Inhibition Induces Cell-Surface Presentation by MOLT-4 Leukemia Cancer Cells of Many Novel and Potentially Antigenic Peptides. Int J Mol Sci, 23(3):(2022) [pubmed]

submitter keyword: ER aminopeptidase 2, ERAP2, Inhibitor, MOLT4, Immunopeptidomics, MHCI, DG011A

Stratos Stratikos
contact affiliation	1 National Centre for Scientific Research “Demokritos”, Agia Paraskevi, Attica, Greece 2 Department of Chemistry, National and Kapodistrian University of Athens, Greece
contact email	estratikos@chem.uoa.gr
lab head
Martina Samiotaki
contact affiliation	Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
contact email	samiotaki@fleming.gr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029895
     1. Label: PRIDE project
     2. Name: An ERAP2 inhibitor induces cell-surface presentation of many new and potentially antigenic peptides by cancer cells