PXD029881

PXD029881 is an original dataset announced via ProteomeXchange.

Title	Olfactomedin-4 improves cutaneous wound healing by promoting skin cell proliferation and migration
Description	Olfactomedin-4 (OLFM4) is an olfactomedin-domain-containing glycoprotein which regulates cell adhesion, proliferation, gastrointestinal inflammation, innate immunity and cancer metastasis. In the present study investigated its role in skin regeneration and wound healing. We found that OLFM4 expression is transiently upregulated in the proliferative phase of cutaneous wound healing in humans as well as in mice. Moreover, a significant increase in OLFM4 expression was detected in the skin of lesional psoriasis, a chronic inflammatory disease characterized by keratinocyte hyperproliferation. In vitro experiments demonstrated that OLFM4 can selectively stimulate keratinocyte proliferation and increase both keratinocyte and fibroblast migration ability. Using proteotransciptomic pathway analysis we revealed that transcription factors POU5F1/OCT4 and ESR1 acted as hubs for OLFM4-dependent signalling in keratinocytes. In vivo experiments utilizing mouse splinted full-thickness cutaneous wound healing model showed that application of recombinant OLFM4 protein can significantly improve wound healing time. Taken together, our results suggest that OLFM4 is a transiently upregulated inflammatory signal that promotes wound healing by supporting the functions of both dermal and epidermal cell compartments.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-19_23:03:08.448.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Viljar Jaks
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-23 07:21:18	ID requested
⏵ 1	2022-05-19 23:03:09	announced

Klaas M, M, ä, emets-Allas K, Heinm, ä, e E, Lagus H, Arak T, Eller M, Kingo K, Kankuri E, Jaks V, Olfactomedin-4 improves cutaneous wound healing by promoting skin cell proliferation and migration through POU5F1/OCT4 and ESR1 signalling cascades. Cell Mol Life Sci, 79(3):157(2022) [pubmed]

submitter keyword: skin regeneration, wound healing, burns, olfactomedin-4, keratinocytes, psoriasis

Viljar Jaks
contact affiliation	Institute of Molecular and Cell Biology, University of Tartu, Riia 23b, 51010, Tartu, Estonia
contact email	viljar.jaks@ut.ee
lab head
Viljar Jaks
contact affiliation	University of Tartu
contact email	viljar.jaks@ut.ee
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD029881

PRIDE project URI

• PRIDE
  1. PXD029881
     1. Label: PRIDE project
     2. Name: Olfactomedin-4 improves cutaneous wound healing by promoting skin cell proliferation and migration