PXD029879

PXD029879 is an original dataset announced via ProteomeXchange.

Title	Evidence for widespread cytoplasmic structuring into mesoscopic condensates
Description	Eukaryotic cytoplasm organizes itself via membrane-bound organelles and membrane-less biomolecular condensates (BMCs). Known BMCs exhibit liquid-like properties and are typically visualized on the scale of ~1 µm. They have been studied mostly by microscopy, examining select individual proteins. Here, we investigate the global organization of native cytoplasm with quantitative proteomics, using differential pressure filtration and dilution experiments. These assays revealed that BMCs form predominantly at the mesoscale of ~100 nm, which we validated via microscopy. Our data indicate that at least 18% of the proteome is organized via such mesoscale BMCs, suggesting that cells widely employ dynamic liquid-like clustering to organize their cytoplasm, at surprisingly small length scales.
HostingRepository	PRIDE
AnnounceDate	2023-11-02
AnnouncementXML	Submission_2023-11-02_11:22:57.745.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	FelixKeber
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-23 02:05:51	ID requested
⏵ 1	2023-11-02 11:22:58	announced

Dataset with its publication pending

submitter keyword: orbitrap fusion lumos, filtration, liquid-liquid phase separation,xenopus laevis

Martin HelmutWühr
contact affiliation	Wühr Lab, Molecular Biology and the Lewis-Sigler Institute for Integrative Genomics, Princeton University, USA
contact email	wuhr@princeton.edu
lab head
FelixKeber
contact affiliation	Princeton University
contact email	fkeber@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD029879

PRIDE project URI

• PRIDE
  1. PXD029879
     1. Label: PRIDE project
     2. Name: Evidence for widespread cytoplasmic structuring into mesoscopic condensates