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PXD029874

PXD029874 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative reactive cysteinome profiling reveals a functional link between ferroptosis and proteasome-mediated degradation.
DescriptionFerroptosis is a unique type of cell death that is hallmarked with the imbalanced redox homeostasis as triggered by iron-dependent lipid peroxidation. Cysteines often play critical roles in proteins to help maintain a healthy cellular environment by dynamically switching between their reduced and oxidized forms, however, how the global redox landscape of cysteinome is perturbed upon ferroptosis remains unknown to date. By using a quantitative chemical proteomic strategy, we systematically profiled the dynamic changes of cysteinome in ferroptotic cells and identified a list of candidate sites whose redox states are precisely regulated under ferroptosis-inducing and rescuing conditions. In particular, C106 of the protein/nucleic acid deglycase DJ-1 acts as an intriguing sensor switch for the ferroptotic condition, whose oxidation results in the disruption of its interaction with the 20S proteasome and leads to a marked activation in the proteasome system. Our chemoproteomic profiling and associated functional studies reveal a novel functional link between ferroptosis and the proteasome-mediated protein degradation and suggest proteasome as a promising target for developing treatment strategies for ferroptosis-related diseases.
HostingRepositoryiProX
AnnounceDate2021-11-23
AnnouncementXMLSubmission_2024-02-26_19:45:41.911.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYankun Wang
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListmethionine oxidation with neutral loss of 64 Da; dimethylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-22 19:28:12ID requested
12021-11-22 19:28:46announced
22023-12-21 22:07:10announced2023-12-22: Update publication information.
32024-02-26 19:45:42announced2023-12-22: Update publication information.
2024-02-27: Update information.
Publication List
Wang Y, Wang C, Quantitative reactive cysteinome profiling reveals a functional link between ferroptosis and proteasome-mediated degradation. Cell Death Differ, 30(1):125-136(2023) [pubmed]
Keyword List
submitter keyword: Ferroptosis, Functional Cysteinome, Quantitative chemical proteomics, DJ-1
Contact List
Chu Wang
contact affiliationPeking University
contact emailchuwang@pku.edu.cn
lab head
Yankun Wang
contact affiliationPeking University
contact emailwangyankun@pku.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI