PXD029874

PXD029874 is an original dataset announced via ProteomeXchange.

Title	Quantitative reactive cysteinome profiling reveals a functional link between ferroptosis and proteasome-mediated degradation.
Description	Ferroptosis is a unique type of cell death that is hallmarked with the imbalanced redox homeostasis as triggered by iron-dependent lipid peroxidation. Cysteines often play critical roles in proteins to help maintain a healthy cellular environment by dynamically switching between their reduced and oxidized forms, however, how the global redox landscape of cysteinome is perturbed upon ferroptosis remains unknown to date. By using a quantitative chemical proteomic strategy, we systematically profiled the dynamic changes of cysteinome in ferroptotic cells and identified a list of candidate sites whose redox states are precisely regulated under ferroptosis-inducing and rescuing conditions. In particular, C106 of the protein/nucleic acid deglycase DJ-1 acts as an intriguing sensor switch for the ferroptotic condition, whose oxidation results in the disruption of its interaction with the 20S proteasome and leads to a marked activation in the proteasome system. Our chemoproteomic profiling and associated functional studies reveal a novel functional link between ferroptosis and the proteasome-mediated protein degradation and suggest proteasome as a promising target for developing treatment strategies for ferroptosis-related diseases.
HostingRepository	iProX
AnnounceDate	2021-11-23
AnnouncementXML	Submission_2024-02-26_19:45:41.911.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yankun Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	methionine oxidation with neutral loss of 64 Da; dimethylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-22 19:28:12	ID requested
1	2021-11-22 19:28:46	announced
2	2023-12-21 22:07:10	announced	2023-12-22: Update publication information.
⏵ 3	2024-02-26 19:45:42	announced	2023-12-22: Update publication information. 2024-02-27: Update information.

submitter keyword: Ferroptosis, Functional Cysteinome, Quantitative chemical proteomics, DJ-1

Chu Wang
contact affiliation	Peking University
contact email	chuwang@pku.edu.cn
lab head
Yankun Wang
contact affiliation	Peking University
contact email	wangyankun@pku.edu.cn
dataset submitter

iProX dataset URI