PXD029874
PXD029874 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative reactive cysteinome profiling reveals a functional link between ferroptosis and proteasome-mediated degradation. |
Description | Ferroptosis is a unique type of cell death that is hallmarked with the imbalanced redox homeostasis as triggered by iron-dependent lipid peroxidation. Cysteines often play critical roles in proteins to help maintain a healthy cellular environment by dynamically switching between their reduced and oxidized forms, however, how the global redox landscape of cysteinome is perturbed upon ferroptosis remains unknown to date. By using a quantitative chemical proteomic strategy, we systematically profiled the dynamic changes of cysteinome in ferroptotic cells and identified a list of candidate sites whose redox states are precisely regulated under ferroptosis-inducing and rescuing conditions. In particular, C106 of the protein/nucleic acid deglycase DJ-1 acts as an intriguing sensor switch for the ferroptotic condition, whose oxidation results in the disruption of its interaction with the 20S proteasome and leads to a marked activation in the proteasome system. Our chemoproteomic profiling and associated functional studies reveal a novel functional link between ferroptosis and the proteasome-mediated protein degradation and suggest proteasome as a promising target for developing treatment strategies for ferroptosis-related diseases. |
HostingRepository | iProX |
AnnounceDate | 2021-11-23 |
AnnouncementXML | Submission_2024-02-26_19:45:41.911.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yankun Wang |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | methionine oxidation with neutral loss of 64 Da; dimethylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-11-22 19:28:12 | ID requested | |
1 | 2021-11-22 19:28:46 | announced | |
2 | 2023-12-21 22:07:10 | announced | 2023-12-22: Update publication information. |
⏵ 3 | 2024-02-26 19:45:42 | announced | 2023-12-22: Update publication information. 2024-02-27: Update information. |
Publication List
Wang Y, Wang C, Quantitative reactive cysteinome profiling reveals a functional link between ferroptosis and proteasome-mediated degradation. Cell Death Differ, 30(1):125-136(2023) [pubmed] |
Keyword List
submitter keyword: Ferroptosis, Functional Cysteinome, Quantitative chemical proteomics, DJ-1 |
Contact List
Chu Wang | |
---|---|
contact affiliation | Peking University |
contact email | chuwang@pku.edu.cn |
lab head | |
Yankun Wang | |
contact affiliation | Peking University |
contact email | wangyankun@pku.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |