PXD029873

PXD029873 is an original dataset announced via ProteomeXchange.

Title	10-HDA reduces the hydroxyl free radical-induced damage to vascular smooth muscle cells by rescuing protein and energy metabolisms
Description	10-hydroxydec-2-enoic acid (10-HDA), a natural agent from royal jelly, could fight against cell and tissue damage, yet the underlying mechanisms are still unexplored. Here, we found the scavenging rate of hydroxyl free radical (•OH) by 2% (g/mL) 10-HDA was more than 20%, which was achieved by a multiple-step oxidization of the −OH group and C=C bond in 10-HDA. Moreover, 10-HDA could enhance the viability of vascular smooth muscle cells (VSMCs) damaged by •OH, indicating its role in reducing the •OH-induced cytotoxicity. The 195 proteins decreased by •OH challenge in VSMCs were up-regulated by 10-HDA rescue, which mainly elevated the activities of protein and energy metabolisms within nucleus and cytoplasm. These evidences indicate that 10-HDA plays key roles in promoting cell survival through antagonizing •OH-induced injury in VSMCs. Our results provide the first solid evidence that 10-HDA scavenging •OH could be a potential pharmacological application in keeping vascular health.
HostingRepository	iProX
AnnounceDate	2021-11-22
AnnouncementXML	Submission_2023-12-21_22:04:30.001.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bin Han
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-22 19:24:48	ID requested
1	2021-11-22 19:25:08	announced
⏵ 2	2023-12-21 22:04:30	announced	2023-12-22: Update information.

Fan P, Sha F, Ma C, Wei Q, Zhou Y, Shi J, Fu J, Zhang L, Han B, Li J, 10-Hydroxydec-2-Enoic Acid Reduces Hydroxyl Free Radical-Induced Damage to Vascular Smooth Muscle Cells by Rescuing Protein and Energy Metabolism. Front Nutr, 9():873892(2022) [pubmed]

submitter keyword: 10-hydroxydec-2-enoic acid, hydroxyl free radical, vascular smooth muscle cell, cytotoxicity, proteomics

Shufa Xu
contact affiliation	Institute of Apicultural Research, Chinese Academy of Agricultural Sciences
contact email	xushufa@caas.cn
lab head
Bin Han
contact affiliation	Institute of Apicultural Research, Chinese Academy of Agricultural Sciences
contact email	hanbin@caas.cn
dataset submitter

iProX dataset URI