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PXD029869

PXD029869 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of RNA helicases that mediate processing of tRNAs into distinct tRNA fragments by LC-MS/MS.
DescriptionSpecific environmental insults cause the limited fragmentation of transfer RNAs (tRNAs) into tRNA-derived small RNAs (tsRNAs), which have been implicated in a wide range of biological processes. tRNA fragmentation results from endonucleolytic activities targeting single-stranded tRNA regions. However, how a tRNA with a single hydrolyzed phosphodiester bond in the anticodon loop (‘nicked’ tRNA) gives rise to distinct tsRNAs remains poorly understood. By utilizing biochemical fractionation of tsRNA-containing ribonucleoprotein complexes (RNPs) coupled with LC-MS/MS, we identified several RNA helicase enzymes that represent putative tRNA/tsRNA processing enzymes. Furthermore, a combination of biochemical and computational approaches revealed that specific RNA helicases indeed bind specific tRNAs with high affinity, as well as process nicked tRNAs into individual tsRNAs. In summary, these findings reveal that tRNA-derived duplexes can be substrates of well-known RNA helicases, thereby expanding their potential for cellular function to the creation of individual tsRNAs during the cellular stress response.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:44:17.450.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDorothea Anrather
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-22 03:24:51ID requested
12023-01-09 03:23:40announced
22023-03-20 04:47:42announced2023-03-20: Updated project metadata.
32023-11-14 08:44:22announced2023-11-14: Updated project metadata.
Publication List
Drino A, K, ö, nig L, Capitanchik C, Sanadgol N, Janisiw E, Rappol T, Vilardo E, Schaefer MR, Identification of RNA helicases with unwinding activity on angiogenin-processed tRNAs. Nucleic Acids Res, 51(3):1326-1352(2023) [pubmed]
Keyword List
submitter keyword: DDX5X,tRNA fragments, DDX3X, tsRNA, RNA helicase enzymes
Contact List
Matthias Schaefer
contact affiliationMedical University of Vienna,Vienna, Austria
contact emailmatthias.schaefer@meduniwien.ac.at
lab head
Dorothea Anrather
contact affiliationMax Perutz Labs, Mass Spectrometry Facility, University of Vienna
contact emaildorothea.anrather@univie.ac.at
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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