PXD029869 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of RNA helicases that mediate processing of tRNAs into distinct tRNA fragments by LC-MS/MS. |
Description | Specific environmental insults cause the limited fragmentation of transfer RNAs (tRNAs) into tRNA-derived small RNAs (tsRNAs), which have been implicated in a wide range of biological processes. tRNA fragmentation results from endonucleolytic activities targeting single-stranded tRNA regions. However, how a tRNA with a single hydrolyzed phosphodiester bond in the anticodon loop (‘nicked’ tRNA) gives rise to distinct tsRNAs remains poorly understood. By utilizing biochemical fractionation of tsRNA-containing ribonucleoprotein complexes (RNPs) coupled with LC-MS/MS, we identified several RNA helicase enzymes that represent putative tRNA/tsRNA processing enzymes. Furthermore, a combination of biochemical and computational approaches revealed that specific RNA helicases indeed bind specific tRNAs with high affinity, as well as process nicked tRNAs into individual tsRNAs. In summary, these findings reveal that tRNA-derived duplexes can be substrates of well-known RNA helicases, thereby expanding their potential for cellular function to the creation of individual tsRNAs during the cellular stress response. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:44:17.450.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dorothea Anrather |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-22 03:24:51 | ID requested | |
1 | 2023-01-09 03:23:40 | announced | |
2 | 2023-03-20 04:47:42 | announced | 2023-03-20: Updated project metadata. |
⏵ 3 | 2023-11-14 08:44:22 | announced | 2023-11-14: Updated project metadata. |
Publication List
Drino A, K, ö, nig L, Capitanchik C, Sanadgol N, Janisiw E, Rappol T, Vilardo E, Schaefer MR, Identification of RNA helicases with unwinding activity on angiogenin-processed tRNAs. Nucleic Acids Res, 51(3):1326-1352(2023) [pubmed] |
Keyword List
submitter keyword: DDX5X,tRNA fragments, DDX3X, tsRNA, RNA helicase enzymes |
Contact List
Matthias Schaefer |
contact affiliation | Medical University of Vienna,Vienna, Austria |
contact email | matthias.schaefer@meduniwien.ac.at |
lab head | |
Dorothea Anrather |
contact affiliation | Max Perutz Labs, Mass Spectrometry Facility, University of Vienna |
contact email | dorothea.anrather@univie.ac.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029869
- Label: PRIDE project
- Name: Identification of RNA helicases that mediate processing of tRNAs into distinct tRNA fragments by LC-MS/MS.