PXD029865

PXD029865 is an original dataset announced via ProteomeXchange.

Title	EFFECTS OF ERYTHRODIOL ON THE ANTIOXIDANT RESPONSE AND PROTEOME OF HepG2 CELLS
Description	Erythrodiol (EO) is a pentacyclic triterpenic alcohol found in the leaf of olive tree and in the olive oil. In this study, we evaluate the nature of cytotoxicity of EO on HepG2 hepatocarcinoma human cells studying the changes induced on cell viability, reactive oxygen species (ROS) production, antioxidant defence systems and proteome. The results obtained reveal that EO markedly decrease HepG2 cell viability without change ROS levels. A significant reduction in the glutathione and NADPH concentrations are produced with selective changes in the activity of antioxidant enzymes (glutathione peroxidase, glutathione reductase, glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase). Proteomics data reveals that 41 and 3 proteins are decreased completely or partially in abundance, respectively; and 29 are completely increased. The functional enrichment analysis showed that an impairment of important metabolic processes and in the nuclear transport of mature mRNA is produced while the biosynthesis of AMP and cell cycle G2/M phase transition are induced. The transcription factors and miRNA implied in this response are also identified
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_06:14:57.122.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carlos Alberto Fuentes Almagro
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-22 02:38:36	ID requested
⏵ 1	2022-02-17 06:14:59	announced

Pe, ñ, as-Fuentes JL, Siles E, Rufino-Palomares EE, P, é, rez-Jim, é, nez A, Reyes-Zurita FJ, Lupi, á, ñ, ez JA, Fuentes-Almagro C, Perag, ó, n-S, á, nchez J, Effects of Erythrodiol on the Antioxidant Response and Proteome of HepG2 Cells. Antioxidants (Basel), 11(1):(2021) [pubmed]

submitter keyword: Erythrodiol, HepG2, cytotoxicity, ROS, antioxidant enzymes, GSH, NADPH, proteome

Juan Peragon Sanchez
contact affiliation	Biochemistry and Molecular Biology Area Departament of Experimental biology University of Jaen (Spain) Campus Las Lagunillas, B3-356 23071, Jaén, Tel.: 953212523
contact email	jperagon@ujaen.es
lab head
Carlos Alberto Fuentes Almagro
contact affiliation	University of Cordoba
contact email	b72fualc@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029865
     1. Label: PRIDE project
     2. Name: EFFECTS OF ERYTHRODIOL ON THE ANTIOXIDANT RESPONSE AND PROTEOME OF HepG2 CELLS