PXD029852

PXD029852 is an original dataset announced via ProteomeXchange.

Title	Mouse Bladder LC-MS after bladder wall injection with Schistosoma haematobium eggs
Description	Urogenital schistosomiasis (caused by Schistosoma haematobium) remains a major public health concern worldwide. In response to parasite egg deposition, the host bladder undergoes gross and molecular morphological changes relevant for disease manifestation. However, limited mechanistic studies to date imply that the molecular mechanisms underlying pathology have not been well-defined. We leveraged a mouse model of urogenital schistosomiasis to perform for the first time, proteome profiling of the early molecular events that occur in the bladder after exposure to S. haematobium eggs, and to elucidate the protein pathways involved in urogenital schistosomiasis-induced pathology. Purified S. haematobium eggs or control vehicle were microinjected into the bladder walls of mice. Mice were sacrificed seven days post-injection and bladder proteins isolated and processed for proteome profiling using mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:41.287.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029852
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Derick Osakunor
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-20 09:50:40	ID requested
1	2022-01-23 08:32:51	announced
2	2022-02-23 07:56:22	announced	2022-02-23: Updated publication reference for DOI(s): doi.org/10.1371/journal.pntd.0010176.
⏵ 3	2023-11-14 08:53:41	announced	2023-11-14: Updated project metadata.

10.6019/PXD029852;

submitter keyword: Bladder

Bladder wall injection

Mice

Proteomics

Schistosoma haematobium

Schistosomiasis, Urogenital schistosomiasis

Michael H. Hsieh
contact affiliation	Division of Urology, Department of Surgery, Sheikh Zayed Institute for Pediatric Surgical Innovation, Children’s National Hospital, Washington, District of Columbia, United States of America. Departments of Urology, Department of Pediatrics, and Department of Microbiology, Immunology, and Tropical Medicine, School of Medicine and Health Sciences, The George Washington University, Washington, District of Columbia, United States of America
contact email	mhsieh@childrensnational.org
lab head
Derick Osakunor
contact affiliation	Children’s National Hospital
contact email	dosakunor@childrensnational.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD029852

PRIDE project URI

• PRIDE
  1. PXD029852
     1. Label: PRIDE project
     2. Name: Mouse Bladder LC-MS after bladder wall injection with Schistosoma haematobium eggs