PXD029851 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A novel structure of GPI anchored proteins NTNG2 |
Description | GPI anchors many proteins to the cell surface. GPI precursor has three mannoses, all of which are modified by ethanolamine-phosphate (EthN-P). It has been believed that EthN-P on the third mannose is always used as a bridge to the protein and EthN-P on the second mannose is removed after GPI is attached to the protein. In fact, several GPI-anchored proteins are not appreciably reduced on cells defective in PIGG, which transfers EthN-P to the second mannose. Nevertheless, mutations in PIGG cause neuronal abnormalities. Here, we show that EthN-P on the second mannose is used as a preferential bridge for several GPI-anchored proteins. Our data modifies the current view of GPI anchors and provides mechanistic basis of PIGG deficiencies. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-31 |
AnnouncementXML | Submission_2022-05-31_06:12:49.690.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD029851 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Yoshiko Murakami |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-20 09:42:57 | ID requested | |
⏵ 1 | 2022-05-31 06:12:50 | announced | |
Publication List
Ishida M, Maki Y, Ninomiya A, Takada Y, Campeau P, Kinoshita T, Murakami Y, Ethanolamine-phosphate on the second mannose is a preferential bridge for some GPI-anchored proteins. EMBO Rep, 23(7):e54352(2022) [pubmed] |
Keyword List
submitter keyword: PIGG PIGB PIGO NTNG2 |
Contact List
Taroh Kinoshita |
contact affiliation | RIMD, Osaka university,Japan |
contact email | tkinoshi@biken.osaka-u.ac.jp |
lab head | |
Yoshiko Murakami |
contact affiliation | Research Institute for Microbial Diseases Osaka University |
contact email | yoshiko@biken.osaka-u.ac.jp |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD029851 |
PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD029851
- Label: PRIDE project
- Name: A novel structure of GPI anchored proteins NTNG2