PXD029850

PXD029850 is an original dataset announced via ProteomeXchange.

Title	A novel structure of GPI anchored proteins-NT5E
Description	GPI anchors many proteins to the cell surface. GPI precursor has three mannoses, all of which are modified by ethanolamine-phosphate (EthN-P). It has been believed that EthN-P on the third mannose is always used as a bridge to the protein and EthN-P on the second mannose is removed after GPI is attached to the protein. In fact, several GPI-anchored proteins are not appreciably reduced on cells defective in PIGG, which transfers EthN-P to the second mannose. Nevertheless, mutations in PIGG cause neuronal abnormalities. Here, we show that EthN-P on the second mannose is used as a preferential bridge for several GPI-anchored proteins. Our data modifies the current view of GPI anchors and provides mechanistic basis of PIGG deficiencies.
HostingRepository	PRIDE
AnnounceDate	2022-05-31
AnnouncementXML	Submission_2022-05-31_06:12:34.451.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029850
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yoshiko Murakami
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-20 09:40:43	ID requested
⏵ 1	2022-05-31 06:12:35	announced

Ishida M, Maki Y, Ninomiya A, Takada Y, Campeau P, Kinoshita T, Murakami Y, Ethanolamine-phosphate on the second mannose is a preferential bridge for some GPI-anchored proteins. EMBO Rep, 23(7):e54352(2022) [pubmed]

submitter keyword: PIGG, PIGO, PIGB, NT5E, CD59

Taroh Kinoshita
contact affiliation	RIMD, Osaka university,Japan
contact email	tkinoshi@biken.osaka-u.ac.jp
lab head
Yoshiko Murakami
contact affiliation	Research Institute for Microbial Diseases Osaka University
contact email	yoshiko@biken.osaka-u.ac.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029850
     1. Label: PRIDE project
     2. Name: A novel structure of GPI anchored proteins-NT5E