PXD029848

PXD029848 is an original dataset announced via ProteomeXchange.

Title	Proteome of Ins1E beta cell line with CerS2 ablation
Description	To gain insight into changes of the proteome of the rat beta cell line Ins1E with and without ablation of CerS2, shotgun proteomics was performed in control cells (transfected withCas9 plasmid without gRNA) and CerS2 KO cells (transfected with Cas9 plasmids with 2 gRNAs, ablating most of the coding exons). Ablation of CerS2 was confirmed by PCR, qPCR and Western Blot.
HostingRepository	PRIDE
AnnounceDate	2022-09-22
AnnouncementXML	Submission_2022-09-22_06:47:47.744.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bengt-Frederik Belgardt
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-20 09:33:42	ID requested
⏵ 1	2022-09-22 06:47:48	announced

Dataset with its publication pending

submitter keyword: Sphingolipids, beta cells, CerS2

Bengt-Frederik Belgardt
contact affiliation	German Diabetes Center (DDZ) Leibniz Center for Diabetes Research at Heinrich Heine University Düsseldorf Auf´m Hennekamp 65 40225 Düsseldorf, Germany phone : +49-(0)-211-33-82-451 Legal body: Deutsche Diabetes-Forschungsgesellschaft e.V.
contact email	bengt.belgardt@ddz.de
lab head
Bengt-Frederik Belgardt
contact affiliation	Institute for Vascular and Islet Cell Biology German Diabetes Center (DDZ) Leibniz Center for Diabetes Research at Heinrich Heine University Düsseldorf Auf´m Hennekamp 65 40225 Düsseldorf, Germany Tel.: +49-(0)-211-33-82-451 www.ddz.de Legal body: Deutsche Diabetes-Forschungsgesellschaft e.V.
contact email	bengt.belgardt@ddz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD029848

PRIDE project URI

• PRIDE
  1. PXD029848
     1. Label: PRIDE project
     2. Name: Proteome of Ins1E beta cell line with CerS2 ablation