PXD029834

PXD029834 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of primary human microvascular endothelial cells (HMVECs) stimulated for 24 h with either 50 �g/mL recombinant Fibrillin-1 C-terminal fragment or 25 ng/ml VEGF-A compared to unstimulated cells.
Description	Mutations in the fibrillin-1 gene result in cardiovascular, ocular, and skeletal abnormalities in Marfan syndrome. Here we show that a fibrillin-1 mutation also alters the neovessel formation. In the mouse retina vascularization model, fibrillin-1 is detected at the basement membrane underlying the angiogenic front and around arterioles. In Fbn1C1041G/+ mice, a model of Marfan disease, the microvasculature is altered at these sites. At the front, endothelial tip-cell sprouting and branching are decreased, and we show that mutant fibrillin-1 delays angiogenesis by affecting Notch signalling. Supplying the growing vasculature of Fbn1C1041G/+ mice with a C-terminal fragment of fibrillin-1 corrects all defects. In vitro, the C-terminal fragment of fibrillin-1 also displays pro-angiogenic activities on microvascular endothelial cell. Our data establish that fibrillin-1 performs essential functions in microvessel formation and integrity and that mutant fibrillin-1-induced defects can be rescued pharmacologically.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:27.784.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-20 08:51:25	ID requested
1	2023-05-31 09:09:34	announced
⏵ 2	2023-11-14 08:48:36	announced	2023-11-14: Updated project metadata.

Alonso F, Dong Y, Li L, Jahjah T, Dupuy JW, Fremaux I, Reinhardt DP, G, é, not E, Fibrillin-1 regulates endothelial sprouting during angiogenesis. Proc Natl Acad Sci U S A, 120(23):e2221742120(2023) [pubmed]

submitter keyword: Endothelial cell, quantitative proteome, angiogenesis

Elisabeth Genot
contact affiliation	Univ. Bordeaux, INSERM, Centre de Recherche cardio-thoracique de Bordeaux, U1045, F-33000 Bordeaux. France
contact email	elisabeth.genot@inserm.fr
lab head
Dupuy Jean-William
contact affiliation	Plateforme Proteome Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD029834

PRIDE project URI

• PRIDE
  1. PXD029834
     1. Label: PRIDE project
     2. Name: Proteomic analysis of primary human microvascular endothelial cells (HMVECs) stimulated for 24 h with either 50 �g/mL recombinant Fibrillin-1 C-terminal fragment or 25 ng/ml VEGF-A compared to unstimulated cells.