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PXD029834

PXD029834 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of primary human microvascular endothelial cells (HMVECs) stimulated for 24 h with either 50 �g/mL recombinant Fibrillin-1 C-terminal fragment or 25 ng/ml VEGF-A compared to unstimulated cells.
DescriptionMutations in the fibrillin-1 gene result in cardiovascular, ocular, and skeletal abnormalities in Marfan syndrome. Here we show that a fibrillin-1 mutation also alters the neovessel formation. In the mouse retina vascularization model, fibrillin-1 is detected at the basement membrane underlying the angiogenic front and around arterioles. In Fbn1C1041G/+ mice, a model of Marfan disease, the microvasculature is altered at these sites. At the front, endothelial tip-cell sprouting and branching are decreased, and we show that mutant fibrillin-1 delays angiogenesis by affecting Notch signalling. Supplying the growing vasculature of Fbn1C1041G/+ mice with a C-terminal fragment of fibrillin-1 corrects all defects. In vitro, the C-terminal fragment of fibrillin-1 also displays pro-angiogenic activities on microvascular endothelial cell. Our data establish that fibrillin-1 performs essential functions in microvessel formation and integrity and that mutant fibrillin-1-induced defects can be rescued pharmacologically.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:48:27.784.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDupuy Jean-William
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-20 08:51:25ID requested
12023-05-31 09:09:34announced
22023-11-14 08:48:36announced2023-11-14: Updated project metadata.
Publication List
Alonso F, Dong Y, Li L, Jahjah T, Dupuy JW, Fremaux I, Reinhardt DP, G, é, not E, Fibrillin-1 regulates endothelial sprouting during angiogenesis. Proc Natl Acad Sci U S A, 120(23):e2221742120(2023) [pubmed]
Keyword List
submitter keyword: Endothelial cell, quantitative proteome, angiogenesis
Contact List
Elisabeth Genot
contact affiliationUniv. Bordeaux, INSERM, Centre de Recherche cardio-thoracique de Bordeaux, U1045, F-33000 Bordeaux. France
contact emailelisabeth.genot@inserm.fr
lab head
Dupuy Jean-William
contact affiliationPlateforme Proteome Bordeaux
contact emailjean-william.dupuy@u-bordeaux.fr
dataset submitter
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Dataset FTP location
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