PXD029834 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of primary human microvascular endothelial cells (HMVECs) stimulated for 24 h with either 50 ï�g/mL recombinant Fibrillin-1 C-terminal fragment or 25 ng/ml VEGF-A compared to unstimulated cells. |
Description | Mutations in the fibrillin-1 gene result in cardiovascular, ocular, and skeletal abnormalities in Marfan syndrome. Here we show that a fibrillin-1 mutation also alters the neovessel formation. In the mouse retina vascularization model, fibrillin-1 is detected at the basement membrane underlying the angiogenic front and around arterioles. In Fbn1C1041G/+ mice, a model of Marfan disease, the microvasculature is altered at these sites. At the front, endothelial tip-cell sprouting and branching are decreased, and we show that mutant fibrillin-1 delays angiogenesis by affecting Notch signalling. Supplying the growing vasculature of Fbn1C1041G/+ mice with a C-terminal fragment of fibrillin-1 corrects all defects. In vitro, the C-terminal fragment of fibrillin-1 also displays pro-angiogenic activities on microvascular endothelial cell. Our data establish that fibrillin-1 performs essential functions in microvessel formation and integrity and that mutant fibrillin-1-induced defects can be rescued pharmacologically. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:48:27.784.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dupuy Jean-William |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-20 08:51:25 | ID requested | |
1 | 2023-05-31 09:09:34 | announced | |
⏵ 2 | 2023-11-14 08:48:36 | announced | 2023-11-14: Updated project metadata. |
Publication List
Alonso F, Dong Y, Li L, Jahjah T, Dupuy JW, Fremaux I, Reinhardt DP, G, é, not E, Fibrillin-1 regulates endothelial sprouting during angiogenesis. Proc Natl Acad Sci U S A, 120(23):e2221742120(2023) [pubmed] |
Keyword List
submitter keyword: Endothelial cell, quantitative proteome, angiogenesis |
Contact List
Elisabeth Genot |
contact affiliation | Univ. Bordeaux, INSERM, Centre de Recherche cardio-thoracique de Bordeaux, U1045, F-33000 Bordeaux. France |
contact email | elisabeth.genot@inserm.fr |
lab head | |
Dupuy Jean-William |
contact affiliation | Plateforme Proteome Bordeaux |
contact email | jean-william.dupuy@u-bordeaux.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029834
- Label: PRIDE project
- Name: Proteomic analysis of primary human microvascular endothelial cells (HMVECs) stimulated for 24 h with either 50 ï�g/mL recombinant Fibrillin-1 C-terminal fragment or 25 ng/ml VEGF-A compared to unstimulated cells.