PXD029831

PXD029831 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Secretome analysis of polarised macrophages derived from induced pluripotent stem cells
Description	Macrophages (Mɸ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated Mɸ can exist in two extreme phenotypes: pro-inflammatory (M1) and anti-inflammatory (M2) Mɸ and these phenotypes can be recapitulated in vitro by using lipopolysaccharide (LPS) plus IFNγ and IL-4, respectively. In the recent years, human induced pluripotent stem cells (iPSC) derived-Mɸ have gained major attention since they are functionally similar to human monocyte derived-Mɸ and are receptive to genome editing. We performed quantitative proteomics of culture supernatants to identify soluble factors released from differentially polarised iPSC-derived Mɸ (iPSDM). Our analysis suggests that the cytokine/chemokine profiles released from polarised iPSDM are similar to monocyte-derived Mɸ.
HostingRepository	PRIDE
AnnounceDate	2022-03-15
AnnouncementXML	Submission_2022-03-15_05:02:53.945.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gavuthami Murugesan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-20 08:45:08	ID requested
⏵ 1	2022-03-15 05:02:54	announced

Publication List

Murugesan G, Davidson L, Jannetti L, Crocker PR, Weigle B, Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells. Biomedicines, 10(2):(2022) [pubmed]

Murugesan G, Davidson L, Jannetti L, Crocker PR, Weigle B, Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells. Biomedicines, 10(2):(2022) [pubmed]

Keyword List

ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project, Stems Cells (B/D-HPP)
submitter keyword: Human, iPSC, secretome, LC-MS

ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project, Stems Cells (B/D-HPP)

submitter keyword: Human, iPSC, secretome, LC-MS

Contact List

Professor Paul Crocker
contact affiliation	Cell Signalling and Immunology Division, School of Life Sciences, University of Dundee
contact email	p.r.crocker@dundee.ac.uk
lab head
Gavuthami Murugesan
contact affiliation	School of Life Sciences, University of Dundee
contact email	gzmurugesan@dundee.ac.uk
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD029831

PRIDE project URI

Repository Record List

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