PXD029824

PXD029824 is an original dataset announced via ProteomeXchange.

Title	Protein kinase Tda1 is essential for Hxk1 and Hxk2 serine 15 phosphorylation
Description	Hexokinase 2 (Hxk2) of Saccharomyces cerevisiae is a dual function hexokinase, acting as a glycolytic enzyme and being involved in the transcriptional regulation of glucose-repressible genes. Relief from glucose repression is accompanied by phosphorylation of Hxk2 at serine 15, which has been attributed to the protein kinase Tda1. To explore the role of Tda1 beyond Hxk2 phosphorylation the proteomic consequences of TDA1 deficiency were investigated by difference gel electrophoresis (2D-DIGE) comparing a wild type and a Δtda1 deletion mutant. Both were grown at 2 % and 0.1 % (w/v) glucose to additionally regard glucose repression/derepression. A total of eight spots exhibiting a minimum 2-fold enhanced or reduced fluorescence upon TDA1 deficiency was detected and identified by mass spectrometry. These include – besides the expected Hxk2 – two proteoforms of hexokinase 1 (Hxk1). Targeted proteomics analyses in conjunction with 2D-DIGE demonstrated that TDA1 is indispensable for Hxk2 and Hxk1 phosphorylation at serine 15. 35 glucose-dependent proteins were identified. To improve spot quantification, a simple method approximating spots as rotationally symmetric solids is presented along with new data on the quantities of Hxk1 and Hxk2 and their serine 15 phosphorylated forms at high and low glucose growth conditions. The Δtda1 deletion mutant exhibited no altered growth comparing high or low glucose conditions or on alternative carbon sources. Also, invertase activity, serving as a reporter for glucose derepression, was not significantly altered. Instead, an involvement of Tda1 in oxidative stress response is suggested.
HostingRepository	PRIDE
AnnounceDate	2022-09-30
AnnouncementXML	Submission_2022-09-30_03:53:46.649.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gunnar Dittmar
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	carbamoylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-20 08:26:57	ID requested
⏵ 1	2022-09-30 03:53:47	announced

Dataset with its publication pending

submitter keyword: PRM, hexokinase

Gunnar Dittmar
contact affiliation	Department of infection and immunity Luxembourg institute of health 1a Rue Thomas Edison 1445 Strassen Luxembourg
contact email	gunnar.dittmar@lih.lu
lab head
Gunnar Dittmar
contact affiliation	LIH
contact email	gunnar.dittmar@lih.lu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029824
     1. Label: PRIDE project
     2. Name: Protein kinase Tda1 is essential for Hxk1 and Hxk2 serine 15 phosphorylation