PXD029785

PXD029785 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Human skin fibroblast proteome in healthy aging
Description	The changes in the proteome of different human tissues with advancing age are poorly characterized. Here, we studied the proteins present in skin fibroblasts collected from 82 healthy individuals across a wide age spectrum (22 to 89 years old) who participated in the GESTALT (Genetic and Epigenetic Signatures of Translational Aging Laboratory Testing) study of the National Institute on Aging, NIH. Proteins were extracted from lysed fibroblasts and subjected to liquid chromatography-mass spectrometry analysis, and the expression levels of 9341 proteins were analyzed by linear regression models. Several differentially expressed proteins were implicated in processes that change with age, including autophagy, scavenging of reactive oxygen species (ROS), ribosome biogenesis, DNA replication, and DNA repair. Changes on these prominent pathways were further assessed using molecular and cell-culture approaches. Our study establishes a framework of the global proteome governing the homeostasis of the aged skin.
HostingRepository	MassIVE
AnnounceDate	2022-04-05
AnnouncementXML	Submission_2022-04-05_10:14:43.836.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ceereena Ubaida-Mohien
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-17 11:13:50	ID requested
⏵ 1	2022-04-05 10:14:44	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: human dermal fibroblasts, aging, proteomics, autophagy, reactive oxygen species, ribosome biogenesis, DNA damage, DNA repair

submitter keyword: human dermal fibroblasts, aging, proteomics, autophagy, reactive oxygen species, ribosome biogenesis, DNA damage, DNA repair

Contact List

Myriam Gorospe
contact affiliation	National Institute on Aging, Intramural Research Program, National Institutes of Health
contact email	myriam-gorospe@nih.gov
lab head
Ceereena Ubaida-Mohien
contact affiliation	National Institute on Aging
contact email	ceereena.ubaida-mohien@nih.gov
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088401/