PXD029782

PXD029782 is an original dataset announced via ProteomeXchange.

Title	Proteomic identification of a gastric tumor ECM signature associated with cancer progression
Description	The extracellular matrix (ECM) plays an undisputable role in tissue homeostasis and its deregulation leads to altered mechanical and biochemical cues that impact cancer development and progression. Herein, we undertook a novel approach to address the role of gastric ECM in tumorigenesis, which remained largely unexplored. By combining decellularization techniques with a high-throughput quantitative proteomics approach, we have performed an extensive characterization of human gastric mucosa, uncovering its composition and distribution among tumor, normal adjacent and normal distant mucosa. Our results revealed a common ECM signature composed of 142 proteins and indicated that gastric carcinogenesis encompasses ECM remodeling through alterations in the abundance of 24 components, mainly basement membrane proteins. Indeed, we could only identify one de novo tumor-specific protein, the collagen alpha-1(X) chain (COL10A1). Functional analysis of the data demonstrated that gastric ECM remodeling favor tumor progression by activating ECM receptors and cellular processes involved in angiogenesis and cell-extrinsic metabolic regulation. By analyzing mRNA expression in an independent GC cohort available at the TGCA, we validated the expression profile of 12 differentially expressed ECM proteins. Importantly, the expression of COL1A2, LOX and LTBP2 significantly correlated with high tumor stage, with LOX and LTBP2 further impacting patient overall survival. Our results contribute for a better understanding of GC biology and highlight the role of core ECM components in gastric carcinogenesis and their clinical relevance as biomarkers of disease prognosis.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_03:36:57.922.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029782
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hugo Osorio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-17 01:18:33	ID requested
⏵ 1	2022-05-20 03:36:58	announced

Moreira AM, Ferreira RM, Carneiro P, Figueiredo J, Os, ó, rio H, Barbosa J, Preto J, Pinto-do-, Ó P, Carneiro F, Seruca R, Proteomic Identification of a Gastric Tumor ECM Signature Associated With Cancer Progression. Front Mol Biosci, 9():818552(2022) [pubmed]

submitter keyword: Extracellular matrix, gastric cancer, matrisome, biomarker, proteomics

Raquel Seruca
contact affiliation	i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal Ipatimup - Instituto de Patologia e Imunologia Molecular da Universidade do Porto, Porto, Portugal
contact email	rseruca@ipatimup.pt
lab head
Hugo Osorio
contact affiliation	i3S - Institute for Research and Innovation in Health, University of Porto
contact email	hosorio@i3s.up.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029782
     1. Label: PRIDE project
     2. Name: Proteomic identification of a gastric tumor ECM signature associated with cancer progression