PXD029781-1

PXD029781 is an original dataset announced via ProteomeXchange.

Title	CerS2 dependent Sphingolipid - protein interactions in rat beta cell line Ins1E
Description	To define the beta cell Sphingolipid-protein interactome, we performed a chemoproteomic screen in which a chemically modified SL precursor (photoactivatable and clickable sphingosine, pacSph) was fed to cells and rapidly incorporated into de novo synthesized SLs. Ultraviolet (UV) irradiation covalently crosslinked cellular SL-protein complexes, allowing for cell lysis under harsh conditions, pulldown and subsequent MS-based identification and quantification of SL interacting proteins. CrispR/Cas9 knockout of the SL specific catabolic enzyme Sgpl1 ensured that only SLs are labelled with pacSph. Furthermore, stable isotope labelling allowed direct comparison of SBPs in several cell lines in the same MS run.
HostingRepository	PRIDE
AnnounceDate	2022-09-22
AnnouncementXML	Submission_2022-09-22_06:35:49.861.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bengt-Frederik Belgardt
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-17 01:11:09	ID requested
⏵ 1	2022-09-22 06:35:50	announced

Dataset with its publication pending

submitter keyword: Sphingolipid, beta cell, interactomics

Bengt-Frederik Belgardt
contact affiliation	German Diabetes Center (DDZ) Leibniz Center for Diabetes Research at Heinrich Heine University Düsseldorf Auf´m Hennekamp 65 40225 Düsseldorf, Germany Tel.: +49-(0)-211-33-82-451
contact email	bengt.belgardt@ddz.de
lab head
Bengt-Frederik Belgardt
contact affiliation	Institute for Vascular and Islet Cell Biology German Diabetes Center (DDZ) Leibniz Center for Diabetes Research at Heinrich Heine University Düsseldorf Auf´m Hennekamp 65 40225 Düsseldorf, Germany Tel.: +49-(0)-211-33-82-451 www.ddz.de Legal body: Deutsche Diabetes-Forschungsgesellschaft e.V.
contact email	bengt.belgardt@ddz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD029781

PRIDE project URI

• PRIDE
  1. PXD029781
     1. Label: PRIDE project
     2. Name: CerS2 dependent Sphingolipid - protein interactions in rat beta cell line Ins1E