PXD029776

PXD029776 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of macrophages derived from induced pluripotent stem cells
Description	Macrophages (Mɸ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated Mɸ can exist in two extreme phenotypes: pro-inflammatory (M1) and anti-inflammatory (M2) Mɸ and these phenotypes can be recapitulated in vitro by using lipopolysaccharide (LPS) plus IFNγ and IL-4, respectively. In the recent years, human induced pluripotent stem cells (iPSC) derived-Mɸ have gained major attention since they are functionally similar to human monocyte derived-Mɸ and are receptive to genome editing. In this study, we used quantitative proteomics to address whether the plasticity of iPSC-derived Mɸ (iPSDM) are similar to human monocyte derived Mɸ. Our analyses suggest that iPSC Mɸ are promising tools to understand Mɸ biology since they exhibit similar polarisation profiles and functions as monocyte-derived Mɸ. We believe this comprehensive proteome data set will be a useful resource in the Mɸ field.
HostingRepository	PRIDE
AnnounceDate	2022-03-15
AnnouncementXML	Submission_2022-03-15_05:07:33.560.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gavuthami Murugesan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-17 00:39:39	ID requested
⏵ 1	2022-03-15 05:07:34	announced

Murugesan G, Davidson L, Jannetti L, Crocker PR, Weigle B, Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells. Biomedicines, 10(2):(2022) [pubmed]

ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project, Stems Cells (B/D-HPP)

submitter keyword: Human, iPSC, macrophages, LC-MS

Professor Paul Crocker
contact affiliation	Division of Cell Signalling and Immunology, School of Life Sciences, University of Dundee, DundeeDD1 5EH, UK.
contact email	p.r.crocker@dundee.ac.uk
lab head
Gavuthami Murugesan
contact affiliation	School of Life Sciences, University of Dundee
contact email	gzmurugesan@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029776
     1. Label: PRIDE project
     2. Name: Proteomic analysis of macrophages derived from induced pluripotent stem cells