PXD029766 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mesenchymal stem cell-derived extracellular vesicles |
Description | Therapeutic benefits of mesenchymal stem/stromal cells (MSCs) are now widely believed to come from their paracrine signalling, i.e. secreted factors such as cytokines, chemokines, and extracellular vesicles (EVs). Cell-free therapy using EVs is an active and emerging field in regenerative medicine. The cellular environment of MSCs is of critical importance when directing paracrine activity. Typical 2D cultivation of stem cells on tissue culture plastic is far removed from the physiological environment of MSCs. The application of 3D cell culture allows MSCs to adapt to their cellular niche environment which, in turn, influences their paracrine signalling activity. In this study we evaluated the impact of 3D MSCs culture on EVs secretion and cargo proteome composition and functional assessment. The outcome highlights critical differences between MSC-EVs obtained from different culture microenvironments, which should be considered when scaling up MSC culture for clinical manufacturing. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-18 |
AnnouncementXML | Submission_2021-11-18_06:47:01.209.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | david greening |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-16 00:48:14 | ID requested | |
⏵ 1 | 2021-11-18 06:47:01 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: mesenchymal stem cell, culture, extracellular vesicles, lung fibrosis |
Contact List
David Greening |
contact affiliation | Baker Heart and Diabetes Institute |
contact email | david.greening@baker.edu.au |
lab head | |
david greening |
contact affiliation | Baker Heart and Diabetes Inst. |
contact email | david.greening@baker.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD029766 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029766
- Label: PRIDE project
- Name: Mesenchymal stem cell-derived extracellular vesicles