PXD029753

PXD029753 is an original dataset announced via ProteomeXchange.

Title	Host protein phosphorylation landscape in A549 cells during influenza A virus entry
Description	Influenza A virus (IAV) is a zoonotic pathogen causing respiratory infections in humans and other mammalian species. Besides the potential to cause pandemics, seasonal IAV causes high medical and economical burden due to 3 to 5 million cases of severe respiratory illness and up to 500,000 deaths every year. Increasing resistance against clinically used anti influenza drugs and an insufficient vaccine protection urge the development of new antiviral strategies to counteract this constant threat to global health. One new approach focuses on cellular factors involved in the IAV life cycle as potential drug targets. Particularly promising are kinases and their target proteins, as kinase inhibitors comprise up to 30% of drug discovery programs in the pharmaceutical industry. In this project, we aim to find suitable candidates for the development of host factor targeted antivirals by using state-of-the-art quantitative phosphoproteomics to reveal the unique phosphoproteome dynamics that occur in the host cell within minutes of IAV infection and enable entry of the virus into its host cell. We identified 3,920 host proteins phosphorylated by infection with avian and seasonal human IAV strains. Among them are known entry factors such as the human epidermal growth factor receptor (EGFR) and members of the phosphoinositid-3-kinase (PI3K) pathway, which validate our approach.
HostingRepository	PRIDE
AnnounceDate	2022-01-13
AnnouncementXML	Submission_2022-01-13_02:47:28.533.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Annika Hunziker
SpeciesList	scientific name: Influenza A virus; NCBI TaxID: 11320; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-15 09:09:07	ID requested
⏵ 1	2022-01-13 02:47:28	announced

Dataset with its publication pending

submitter keyword: Human, Phosphoproteomics, influenza A virus

Silke Stertz
contact affiliation	Institute of Medical Virology, University of Zurich
contact email	stertz.silke@virology.uzh.ch
lab head
Annika Hunziker
contact affiliation	University of Zurich
contact email	hunziker.annika@virology.uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD029753

PRIDE project URI

• PRIDE
  1. PXD029753
     1. Label: PRIDE project
     2. Name: Host protein phosphorylation landscape in A549 cells during influenza A virus entry