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PXD029751

PXD029751 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitletRNA synthetases are recruited to yeast ribosomes by rRNA expansion segment 7L but do not require association for func-tionality
DescriptionRibosomes expanded during evolution on the protein and rRNA level. The expanded regions do not change the active sites of ribosomes and thus do not alter the peptide bond formation reaction catalysed by the ribosome. To what purpose these expansions were selected is not fully understood. Here, we deleted one rRNA expansion segment (ES7La) and addressed physiological consequences. We found a mild ribosome biogenesis defect accompanied by slightly reduced translation. On a molecular level, ribosomes displayed reduced association to three aminoacyl-tRNA synthetases. Loss of ribosome binding however did not alter synthetase activity suggesting, that tRNAs are not channelled for enhanced local translation in the case of these synthetases.
HostingRepositoryPRIDE
AnnounceDate2022-02-16
AnnouncementXMLSubmission_2022-02-16_06:33:23.390.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRobert Rauscher
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-15 07:05:32ID requested
12022-02-16 06:33:24announced
Publication List
Krauer N, Rauscher R, Polacek N, tRNA Synthetases Are Recruited to Yeast Ribosomes by rRNA Expansion Segment 7L but Do Not Require Association for Functionality. Noncoding RNA, 7(4):(2021) [pubmed]
Keyword List
submitter keyword: Ribosome biology, rRNA expansion segments, aminoacyl-tRNA synthetases, translation regulation, Yeast
Contact List
Norbert Polacek
contact affiliationDepartment for Chemistry, Biochemistry and pharmaceutical sciences University Of Bern Switzerland
contact emailnorbert.polacek@unibe.ch
lab head
Robert Rauscher
contact affiliationUniversity of Bern
contact emailag.polacek@gmail.com
dataset submitter
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Dataset FTP location
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