PXD029751

PXD029751 is an original dataset announced via ProteomeXchange.

Title	tRNA synthetases are recruited to yeast ribosomes by rRNA expansion segment 7L but do not require association for func-tionality
Description	Ribosomes expanded during evolution on the protein and rRNA level. The expanded regions do not change the active sites of ribosomes and thus do not alter the peptide bond formation reaction catalysed by the ribosome. To what purpose these expansions were selected is not fully understood. Here, we deleted one rRNA expansion segment (ES7La) and addressed physiological consequences. We found a mild ribosome biogenesis defect accompanied by slightly reduced translation. On a molecular level, ribosomes displayed reduced association to three aminoacyl-tRNA synthetases. Loss of ribosome binding however did not alter synthetase activity suggesting, that tRNAs are not channelled for enhanced local translation in the case of these synthetases.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_06:33:23.390.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Rauscher
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-15 07:05:32	ID requested
⏵ 1	2022-02-16 06:33:24	announced

Krauer N, Rauscher R, Polacek N, tRNA Synthetases Are Recruited to Yeast Ribosomes by rRNA Expansion Segment 7L but Do Not Require Association for Functionality. Noncoding RNA, 7(4):(2021) [pubmed]

submitter keyword: Ribosome biology, rRNA expansion segments, aminoacyl-tRNA synthetases, translation regulation, Yeast

Norbert Polacek
contact affiliation	Department for Chemistry, Biochemistry and pharmaceutical sciences University Of Bern Switzerland
contact email	norbert.polacek@unibe.ch
lab head
Robert Rauscher
contact affiliation	University of Bern
contact email	ag.polacek@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD029751

PRIDE project URI

• PRIDE
  1. PXD029751
     1. Label: PRIDE project
     2. Name: tRNA synthetases are recruited to yeast ribosomes by rRNA expansion segment 7L but do not require association for func-tionality