PXD029751 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | tRNA synthetases are recruited to yeast ribosomes by rRNA expansion segment 7L but do not require association for func-tionality |
Description | Ribosomes expanded during evolution on the protein and rRNA level. The expanded regions do not change the active sites of ribosomes and thus do not alter the peptide bond formation reaction catalysed by the ribosome. To what purpose these expansions were selected is not fully understood. Here, we deleted one rRNA expansion segment (ES7La) and addressed physiological consequences. We found a mild ribosome biogenesis defect accompanied by slightly reduced translation. On a molecular level, ribosomes displayed reduced association to three aminoacyl-tRNA synthetases. Loss of ribosome binding however did not alter synthetase activity suggesting, that tRNAs are not channelled for enhanced local translation in the case of these synthetases. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-16 |
AnnouncementXML | Submission_2022-02-16_06:33:23.390.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Robert Rauscher |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-15 07:05:32 | ID requested | |
⏵ 1 | 2022-02-16 06:33:24 | announced | |
Publication List
Krauer N, Rauscher R, Polacek N, tRNA Synthetases Are Recruited to Yeast Ribosomes by rRNA Expansion Segment 7L but Do Not Require Association for Functionality. Noncoding RNA, 7(4):(2021) [pubmed] |
Keyword List
submitter keyword: Ribosome biology, rRNA expansion segments, aminoacyl-tRNA synthetases, translation regulation, Yeast |
Contact List
Norbert Polacek |
contact affiliation | Department for Chemistry, Biochemistry and pharmaceutical sciences University Of Bern Switzerland |
contact email | norbert.polacek@unibe.ch |
lab head | |
Robert Rauscher |
contact affiliation | University of Bern |
contact email | ag.polacek@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD029751
- Label: PRIDE project
- Name: tRNA synthetases are recruited to yeast ribosomes by rRNA expansion segment 7L but do not require association for func-tionality