PXD029750

PXD029750 is an original dataset announced via ProteomeXchange.

Title	Analysis of CD2 interactome in primary murine CD4+ T cells
Description	CD2 is a receptor expressed on T cells and when engaged, it promotes T cell activation. However the mechanisms and molecular partners of CD2 that mediate T cell activation remain unclear. Here we used AP-MS to determine the interactome of CD2 prior and upon its engagement. For this purpose, we used a knockin mouse expressing an endogenous One Step Tag (OST) version of the CD2 molecule. Affinity purification of the OST tagged protein was performed using Streptactin beads, from T cells left non-stimulated, or stimulated for 1min or 5min with anti-CD2 antibodies. Each AP-MS purification was associated with a corresponding control (purification from WT CD4+ T cells, cultured and stimulated in the same conditions). The number of replicate biological experiments was n=3 for all conditions.
HostingRepository	PRIDE
AnnounceDate	2022-07-12
AnnouncementXML	Submission_2022-07-12_01:34:58.626.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anne Gonzalez de Peredo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-15 06:52:07	ID requested
⏵ 1	2022-07-12 01:34:58	announced

Dataset with its publication pending

submitter keyword: CD2, interactome, primary CD4+ T cell, mouse, OST tag, AP-MS

Odile Schiltz
contact affiliation	Institute of Pharmacology and Structural Biology, CNRS, Toulouse, France
contact email	Odile.Schiltz@ipbs.fr
lab head
Anne Gonzalez de Peredo
contact affiliation	CNRS/IPBS
contact email	gonzalez@ipbs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029750
     1. Label: PRIDE project
     2. Name: Analysis of CD2 interactome in primary murine CD4+ T cells