PXD029749

PXD029749 is an original dataset announced via ProteomeXchange.

Title	A chemoproteomics approach to profile phospholipase D-derived phosphatidyl alcohol interactions
Description	Alcohol consumption leads to formation of phosphatidylethanol (PEth) via the transphosphatidylation activity of phospholipase D (PLD) enzymes. Though this non-natural phospholipid routinely serves as a biomarker of chronic alcoholism, its pathophysiological roles remain unknown. We use a minimalist diazirene alkyne alcohol as an ethanol surrogate to generate clickable, photoaffinity lipid reporters of PEth localization and lipid–protein interactions via PLD-mediated transphosphatidylation. Using click chemistry tagging, enrichment, and proteomics analysis, we identified the single-pass transmembrane protein basigin/CD147 as a high-confidence interaction partner of this photoaffinity lipid reporter. Here we perform in-cell photocrosslinking, followed by anti-FLAG affinity enrichment of FLAG-tagged basigin, to map the crosslinking sites and determine that the PAL lipid crosslinks to a C-terminal peptide of basigin-FLAG. This study provides a view of the molecular interactions of phosphatidyl alcohols and points to future work to connect such interactions to potential pathophysiological roles of PEth.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:19:14.678.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029749
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christina Woo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-15 06:47:24	ID requested
1	2024-01-04 03:32:34	announced
⏵ 2	2024-10-22 06:19:15	announced	2024-10-22: Updated project metadata.

10.6019/PXD029749;

Yu W, Lin Z, Woo CM, Baskin JM, A Chemoproteomics Approach to Profile Phospholipase D-Derived Phosphatidyl Alcohol Interactions. ACS Chem Biol, 17(12):3276-3283(2022) [pubmed]

10.1021/acschembio.1c00584;

submitter keyword: CuAAC, basigin, phosphatidyl ethanol, phosphatidic acid, diazirene, photoaffinity label, click chemistry,Phospholipase D, phosphatidyl alcohol

Christina May Woo
contact affiliation	Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA
contact email	cwoo@chemistry.harvard.edu
lab head
Christina Woo
contact affiliation	Harvard University
contact email	cwoo@chemistry.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD029749

PRIDE project URI

• PRIDE
  1. PXD029749
     1. Label: PRIDE project
     2. Name: A chemoproteomics approach to profile phospholipase D-derived phosphatidyl alcohol interactions