PXD029745

PXD029745 is an original dataset announced via ProteomeXchange.

Title	NAD metabolism modulates mitochondrial function and inflammation and prevents progression of diabetic kidney disease
Description	Background: Diabetes mellitus is the leading cause of cardiovascular and renal disease in the United States. In spite of all of the beneficial interventions implemented in patients with diabetes, there remains a need for additional therapeutic targets in diabetic kidney disease (DKD). Mitochondrial dysfunction and inflammation are increasingly recognized as important causes of the development and progression of DKD. However, the molecular connection between mitochondrial function, inflammation, and fibrosis remains to be elucidated. Methods: In the present studies we tested the hypothesis that enhancing NAD metabolism could increase mitochondrial sirtuin 3 (SIRT3) activity, improve mitochondrial function, decrease mitochondrial DNA damage, and prevent inflammation and progression of DKD. Results: We found that treatment of db-db mice with type 2 diabetes with nicotinamide riboside (NR) prevented albuminuria, increased urinary KIM1 excretion, and several parameters of DKD. These effects were associated with increased SIRT3 activity, improved mitochondrial function, and decreased inflammation at least in part via inhibiting the activation of the cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) signaling pathway. Conclusions: NR supplementation boosted the NAD metabolism to modulate mitochondrial function and inflammation and prevent progression of diabetic kidney disease.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:59:36.494.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Panov
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-15 06:26:54	ID requested
1	2023-08-02 09:27:34	announced
2	2023-11-14 06:54:26	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:59:36	announced	2024-10-22: Updated project metadata.

10.1016/J.JBC.2023.104975;

submitter keyword: NR, kidney,diabetes, dbdb

Moshe Levi
contact affiliation	Department of Biochemistry and Molecular & Cellular Biology, Georgetown University, Washington, DC
contact email	moshe.levi@georgetown.edu
lab head
Julia Panov
contact affiliation	University of Haifa
contact email	juliapanov.uni@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD029745

PRIDE project URI

• PRIDE
  1. PXD029745
     1. Label: PRIDE project
     2. Name: NAD metabolism modulates mitochondrial function and inflammation and prevents progression of diabetic kidney disease