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PXD029727

PXD029727 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleConservation and divergence of myelin proteome profiles between humans and mice
DescriptionWhile myelin is commonly assessed in mice as a model for humans, it remained unclear to which extent their myelin protein composition is similar. We analyzed the proteome of myelin biochemically purified from human white matter by two steps of discontinuous sucrose density gradient centrifugation intermitted by osmotic shocks. We utilized a data-independent acquisition (DIA) workflow with alternating low and elevated energy (MSE) and an ion mobility-enhanced version thereof (referred to as UDMSE) to achieve both, a correct quantification of exceptionally abundant myelin proteins and a comprehensive coverage of the myelin proteome. Label-free protein quantification revealed that the relative abundance of the structural myelin proteins PLP, MBP, CNP and SEPTIN8 correlates well with that in c57Bl/6N-mice. Conversely, multiple other proteins were identified exclusively or predominantly in human or mouse myelin. Species-dependent diversity of myelin protein composition can be instructive when translating from mouse models to humans.
HostingRepositoryPRIDE
AnnounceDate2022-05-16
AnnouncementXMLSubmission_2022-05-16_04:28:19.257.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOlaf Jahn
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-12 07:33:28ID requested
12022-05-16 04:28:20announced
Publication List
Gargareta VI, Reuschenbach J, Siems SB, Sun T, Piepkorn L, Mangana C, Sp, ä, te E, Goebbels S, Huitinga I, M, ö, bius W, Nave KA, Jahn O, Werner HB, Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice. Elife, 11():(2022) [pubmed]
Keyword List
submitter keyword: Oligodendrocyte, myelin proteome, central nervous system (CNS), human post mortem brain tissue, autopsy, label-free proteomics, mass spectrometry, translation
Contact List
Prof. Dr. Olaf Jahn
contact affiliationProteomics Group Max Planck Institute of Experimental Medicine Hermann-Rein-Str. 3 37075 Goettingen, Germany Tel: +49-551-3899313 Fax: +49-551-3899323
contact emailjahn@em.mpg.de
lab head
Olaf Jahn
contact affiliationProteomics Group, Max-Planck-Institute of Experimental Medicine, Göttingen
contact emailjahn@em.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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