PXD029711

PXD029711 is an original dataset announced via ProteomeXchange.

Title	Sulfamethoxazole triggers specific microbial activities under aerobic heterotrophic conditions: A metaproteomic approach
Description	The global sanitary crisis derived from antibiotic multi-resistant bacteria entails the need to reduce sulfamethoxazole (SMX) concentrations in wastewater treatment plants (WWTPs). The key microorganisms and the biotransformation mechanisms leading to SMX removal remain incompletely characterized, particularly under aerobic heterotrophic conditions, which are becoming increasingly relevant in the design of novel, more energy-efficient, WWTPs. In this study, sequential batch reactors were inoculated with activated sludge, operated in heterotrophic conditions and spiked with six different initial SMX concentrations ranging between 0 and 2000 µg L-1. The goal was to determine the influence of SMX in the microbiome and its enzymatic expression through genomic, metaproteomic and transformation product analyses. The results allowed us to identify the metabolite 2,4(1H,3H)-pteridinedione-SMX (PtO-SMX), pointing to the role of the pterin-conjugation pathway in the biotransformation of SMX. Additionally, at increased SMX concentrations, through metaproteomics and 16S rRNA gene sequencing, it was determined a higher abundance of the genus Corynebacterium and a differential expression of five enzymes involved in its central metabolism, suggesting the relevant role of this bacteria to mitigate SMX risks.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:54:11.289.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029711
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Valentina Calamia
SpeciesList	scientific name: environmental samples
ModificationList	acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-12 06:07:14	ID requested
1	2022-09-20 08:00:10	announced
⏵ 2	2023-11-14 08:54:11	announced	2023-11-14: Updated project metadata.

10.6019/PXD029711;

submitter keyword: Activated sludge

antibiotics

biotransformation

enzymes

micropollutants

proteome

transformation products

wastewater

Alba Trueba Santiso
contact affiliation	Biogroup - Group of Environmental Biotechnology Department of Chemical Engineering . Universidade de Santiago de Compostela
contact email	albamaria.trueba@usc.es
lab head
Valentina Calamia
contact affiliation	INIBIC
contact email	vcalami@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029711
     1. Label: PRIDE project
     2. Name: Sulfamethoxazole triggers specific microbial activities under aerobic heterotrophic conditions: A metaproteomic approach