PXD029675 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chemical Probing Discovers New Insights into the Native Assembly State of a Bacterial Microcompartment |
Description | Microcompartments (BMCs) are widespread in bacteria and are used for a variety of metabolic purposes, including catabolism of host metabolites. A suite of proteins self-assembles into the shell and cargo layers of BMCs. However, the native assembly state of these large complexes remains to be elucidated. Herein, chemical probes were used to discover structural features of a native BMC. While the exterior could be demarcated with fluorophores, the interior was unexpectedly permeable, suggesting the shell layer may be more dynamic than thought. This allowed access to cross-linking chemical probes, which were analyzed for discovery of the protein interactome. These cross-links revealed a complex multivalent network among cargo proteins that contained encapsulation peptides and demonstrated that the shell layer follows discreet rules in its assembly. These results are consistent overall with a model where biomolecular condensation drives interactions of cargo proteins prior to envelopment by shell layer proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-19 |
AnnouncementXML | Submission_2022-08-18_21:38:43.360.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Conor Jenkins |
SpeciesList | scientific name: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2; NCBI TaxID: 99287; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-11 04:51:34 | ID requested | |
⏵ 1 | 2022-08-18 21:38:44 | announced | |
Publication List
Trettel DS, Resager W, Ueberheide BM, Jenkins CC, Winkler WC, Chemical probing provides insight into the native assembly state of a bacterial microcompartment. Structure, 30(4):537-550.e5(2022) [pubmed] |
Keyword List
submitter keyword: Bacterial microcompartment, chemical modification, dynamic, cross-linking, interactome |
Contact List
Wade C. Winkler |
contact affiliation | The University of Maryland Department of Cell Biology and Molecular Genetics |
contact email | wwinkler@umd.edu |
lab head | |
Conor Jenkins |
contact affiliation | US Army DEVCOM CBC |
contact email | conor.c.jenkins.civ@army.mil |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD029675 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029675
- Label: PRIDE project
- Name: Chemical Probing Discovers New Insights into the Native Assembly State of a Bacterial Microcompartment