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PXD029675

PXD029675 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChemical Probing Discovers New Insights into the Native Assembly State of a Bacterial Microcompartment
DescriptionMicrocompartments (BMCs) are widespread in bacteria and are used for a variety of metabolic purposes, including catabolism of host metabolites. A suite of proteins self-assembles into the shell and cargo layers of BMCs. However, the native assembly state of these large complexes remains to be elucidated. Herein, chemical probes were used to discover structural features of a native BMC. While the exterior could be demarcated with fluorophores, the interior was unexpectedly permeable, suggesting the shell layer may be more dynamic than thought. This allowed access to cross-linking chemical probes, which were analyzed for discovery of the protein interactome. These cross-links revealed a complex multivalent network among cargo proteins that contained encapsulation peptides and demonstrated that the shell layer follows discreet rules in its assembly. These results are consistent overall with a model where biomolecular condensation drives interactions of cargo proteins prior to envelopment by shell layer proteins.
HostingRepositoryPRIDE
AnnounceDate2022-08-19
AnnouncementXMLSubmission_2022-08-18_21:38:43.360.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterConor Jenkins
SpeciesList scientific name: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2; NCBI TaxID: 99287;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-11 04:51:34ID requested
12022-08-18 21:38:44announced
Publication List
Trettel DS, Resager W, Ueberheide BM, Jenkins CC, Winkler WC, Chemical probing provides insight into the native assembly state of a bacterial microcompartment. Structure, 30(4):537-550.e5(2022) [pubmed]
Keyword List
submitter keyword: Bacterial microcompartment, chemical modification, dynamic, cross-linking, interactome
Contact List
Wade C. Winkler
contact affiliationThe University of Maryland Department of Cell Biology and Molecular Genetics
contact emailwwinkler@umd.edu
lab head
Conor Jenkins
contact affiliationUS Army DEVCOM CBC
contact emailconor.c.jenkins.civ@army.mil
dataset submitter
Full Dataset Link List
Dataset FTP location
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