PXD029672

PXD029672 is an original dataset announced via ProteomeXchange.

Title	Structural Alternation in Heat Shock Proteins of Activated Macrophages
Description	The inflammatory response of macrophages is an orderly and complex process under strict reg-ulation accompanied by drastic changes in morphology and functions. It is predicted that pro-teins will undergo structural changes during these finely regulated processes. However, changes in structural proteome in macrophages during the inflammatory response remain poorly char-acterized. In the present study, we applied limited proteolysis coupled mass spectrometry (LiP-MS) to identify proteome-wide structural changes in lipopolysaccharide (LPS)-activated macrophages. We identified 386 structure-specific proteolytic fingerprints from 230 proteins. Using GO molecular function enrichment, we discovered that proteins with altered structures were enriched into unfolded protein binding and protein folding chaperone, in which HSP60 was ranked as the most changed protein. We verified the structural changes in HSP60 by using cellular thermal shift assay (CETSA) and native CETSA. Our results showed that the thermal sta-bility of HSP60 was enhanced in activated macrophages and formed a HSP10-less complex. In conclusion, we demonstrate that in situ structural systems biology is an effective method to characterize proteomic structural changes and reveal that the structures of chaperone proteins vary significantly in macrophage activation.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_03:59:35.877.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenhao Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-11 04:32:43	ID requested
⏵ 1	2022-02-17 03:59:36	announced

Zhang W, Wei Y, Zhang H, Liu J, Zong Z, Liu Z, Zhu S, Hou W, Chen Y, Deng H, Structural Alternation in Heat Shock Proteins of Activated Macrophages. Cells, 10(12):(2021) [pubmed]

submitter keyword: Structural proteomics

RAW264.7

LPS

Lip-MS

HSP60

Haiteng Deng
contact affiliation	MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systematic Biology, School of Life Sciences, Tsinghua University, Beijing, China.100084
contact email	dht@mail.tsinghua.edu.cn
lab head
Wenhao Zhang
contact affiliation	Tsinghua University
contact email	zwh17@mails.tsinghua.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029672
     1. Label: PRIDE project
     2. Name: Structural Alternation in Heat Shock Proteins of Activated Macrophages