PXD029668

PXD029668 is an original dataset announced via ProteomeXchange.

Title	Comparative investigations on surfactin as an antimicrobial metabolite
Description	Surfactin is described as one of the most powerful biosurfactants and is natively produced by Ba-cillus subtilis in notable quantities. In addition to other industrially relevant characteristics, anti-microbial properties were highlighted for surfactin. To investigate this property, stress ap-proaches were carried out with biotechnologically established strains of Corynebacterium glutami-cum, Bacillus subtilis, Escherichia coli and Pseudomonas putida with the highest possible amounts of surfactin. Contrary to the popular opinion, growth-reducing effects were only detectable for B. subtilis and E. coli at the higher gram-per-litre scale, while other antimicrobial biosurfactants, such as rhamnolipids and sophorolipids, showed significantly stronger effects on bacterial growth. As the addition of high amounts of surfactin in defined mineral salt medium reduced the cell growth of B. subtilis by about 40%, the initial stress response at the protein level was ana-lyzed by mass spectrometry, showing an induction of stress proteins under the control of the al-ternative sigma factors σB and σW as well as the activation of LiaRS two-component system. Overall, growth-reducing effects could be demonstrated after addition of high amounts of sur-factin at a high gram-per-litre scale, while absolutely no effects could be detected for the exem-plary bacterial strains C. glutamicum and P. putida, challenging the general claim of the antimi-crobial properties of surfactin.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-19_23:38:13.884.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jens Pfannstiel
SpeciesList	scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308; scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Pseudomonas putida KT2440; NCBI TaxID: 160488; scientific name: Corynebacterium glutamicum ATCC 13032; NCBI TaxID: 196627;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-11 04:19:06	ID requested
⏵ 1	2022-05-19 23:38:14	announced

Lilge L, Ersig N, Hubel P, Aschern M, Pillai E, Klausmann P, Pfannstiel J, Henkel M, Morabbi Heravi K, Hausmann R, and Other Bacterial Model Organisms in the Absence of Synergistic Metabolites. Microorganisms, 10(4):(2022) [pubmed]

submitter keyword: Bacillus subtilis, surfactin, biosurfactants, stress response, cell adaptation, proteomics

Rudolf Hausmann
contact affiliation	Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology (150), University of Hohenheim, Fruwirthstr. 12, 70599 Stuttgart, Germany
contact email	rudolf.hausmann@uni-hohenheim.de
lab head
Jens Pfannstiel
contact affiliation	University of Hohenheim
contact email	Jens.Pfannstiel@uni-hohenheim.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029668
     1. Label: PRIDE project
     2. Name: Comparative investigations on surfactin as an antimicrobial metabolite