PXD029659

PXD029659 is an original dataset announced via ProteomeXchange.

Title	LYSET/TMEM251 KO cellular and lysosomal proteomes from MIA PaCa-2 cells
Description	Proteome analysis of post-nuclear supernatants (PNS) and enriched lysosomal fractions from LYSET/TMEM251 knockout and control MIA PaCa-2 cells. Lysosomes were loaded with iron nanoparticles (DexoMAG C, Liquids Research). PNS were generated from cell homogenates and used as input to enrich lysosomal fractions over magnetic LS MACS columns (Miltenyi).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:39.515.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wilhelm Palm
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-10 07:57:59	ID requested
1	2022-09-29 01:56:40	announced
⏵ 2	2023-11-14 08:53:39	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: PNS, lysosomes,human, pancreatic cancer

Wilhelm Palm
contact affiliation	Cell Signaling and Metabolism Group German Cancer Research Center (DKFZ)
contact email	w.palm@dkfz-heidelberg.de
lab head
Wilhelm Palm
contact affiliation	German Cancer Research Center (DKFZ)
contact email	w.palm@dkfz-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD029659

PRIDE project URI

• PRIDE
  1. PXD029659
     1. Label: PRIDE project
     2. Name: LYSET/TMEM251 KO cellular and lysosomal proteomes from MIA PaCa-2 cells