PXD029655 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic discovery of chemical probes that perturb protein complexes in human cells |
Description | Most proteins in the human proteome lack chemical probes, and several large-scale and generalizable small-molecule binding assays have been introduced to address this problem. How compounds discovered in such “binding-first” assays affect protein function, nonetheless, often remains unclear. Here, we describe a “function-first” proteomic strategy that uses size exclusion chromatography (SEC) to assess the global impact of electrophilic compounds on protein complexes in human cells. Integrating the SEC data with cysteine-directed activity-based protein profiling identifies changes in protein-protein interactions that are caused by site-specific liganding events, including the stereoselective engagement of cysteines in PSME1 and SF3B1 that disrupt the PA28 proteasome regulatory complex and stabilize a dynamic state of the spliceosome, respectively. Our findings thus show how multidimensional proteomic analysis of focused libraries of electrophilic compounds can expedite the discovery of chemical probes with site-specific functional effects on protein complexes in human cells. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:58:51.940.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jarrett Remsberg |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-10 07:55:08 | ID requested | |
1 | 2023-04-20 13:38:47 | announced | |
⏵ 2 | 2023-11-14 08:58:52 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Human, size-exclusion, chemoproteomics, immunopeptidomics, covalent ligands |
Contact List
Benjamin F. Cravatt |
contact affiliation | Professor, Gilula Chair in Chemical Biology, The Department of Chemistry, Skaggs Institute for Chemical Biology, The Scripps Research Institute 10550 North Torrey Pines Rd, La Jolla, CA 92037 |
contact email | cravatt@scripps.edu |
lab head | |
Jarrett Remsberg |
contact affiliation | Scripps Research Institute |
contact email | remsberg@scripps.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD029655 |
PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD029655
- Label: PRIDE project
- Name: Proteomic discovery of chemical probes that perturb protein complexes in human cells