PXD029650

PXD029650 is an original dataset announced via ProteomeXchange.

Title	Involvement of low molecular weight tyrosine acid phosphatase in abscisic acid signaling in Arabidopsis
Description	Low molecular weight protein tyrosine phosphatase (LWM-PTP) is highly conserved tyrosine phosphatase from plants to animals. The function and putative targets of plant LWM-PTP homolog is not reported yet. Here, we revealed that Arabidopsis LWM-PTP homolog APH is a functional tyrosine phosphatase. APH participates in ABA signaling and regulates the ABA-responsive genes by regulates the tyrosine phosphorylation of multiple splicing factors and other transcriptional regulators. APH may also target RAF9, a member of B2 and B3 RAF kinases that activates SnRK2s, the key components in ABA-receptor coupled core signaling pathway. We preformed genetic analysis and found the aph mutants are hyposensitive to ABA in post-germination growth. We also performed an anti-phosphotyrosine antibody-based phosphoproteomics and studied the global change of phosphotyrosine in response to the ABA and APH. Hundreds of proteins, include SR45 and RAF9, are identified putative targets of APH. Consistent with it, aph mutants showed an ABA hyposensitive phenotype and altered expression of ABA-highly-responsive genes. Our results reveal a crucial function of APH in regulating tyrosine phosphorylation and ABA signaling in model plant Arabidopsis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:28:37.551.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rongxia Li
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-10 07:51:48	ID requested
1	2022-10-14 00:23:10	announced
⏵ 2	2023-11-14 08:28:38	announced	2023-11-14: Updated project metadata.

Du Y, Xie S, Wang Y, Ma Y, Jia B, Liu X, Rong J, Li R, Zhu X, Song CP, Tao WA, Wang P, Low molecular weight protein phosphatase APH mediates tyrosine dephosphorylation and ABA response in Arabidopsis. Stress Biol, 2(1):23(2022) [pubmed]

ProteomeXchange project tag: Antibodies (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: homolog, tyrosine phosphorylation,Low molecular weight protein tyrosine phosphatase

Pengcheng Wang
contact affiliation	Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China
contact email	pcwang@psc.ac.cn
lab head
Rongxia Li
contact affiliation	Key lab of systems biology
contact email	rongxiali@sibs.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029650
     1. Label: PRIDE project
     2. Name: Involvement of low molecular weight tyrosine acid phosphatase in abscisic acid signaling in Arabidopsis